UniProt Accession

 KLF1_HUMAN; Q13351; Q6PIJ5; Q92899 

Genbank Protein ID

 U37106; U65404; AD000092; BC033580 

Genbank Nucleotide ID

 AAC50562.1; AAC51108.1; AAB51173.1; AAH33580.1 

Protein Name

 Krueppel-like factor 1 

Protein Synonyms/Alias

 Erythroid krueppel-like transcription factor; EKLF 

Gene Name

 KLF1 

Gene Synonyms/Alias

 EKLF 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
265	IAETAPSKRGRRSWA	acetylation	[1]
274	GRRSWARKRQAAHTC	acetylation	[1, 2]
288	CAHPGCGKSYTKSSH	acetylation	[2]

Reference

 [1] Acetylation and modulation of erythroid Krüppel-like factor (EKLF) activity by interaction with histone acetyltransferases.
 Zhang W, Bieker JJ.
 Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):9855-60. [PMID: 9707565]
 [2] Site-specific acetylation by p300 or CREB binding protein regulates erythroid Krüppel-like factor transcriptional activity via its interaction with the SWI-SNF complex.
 Zhang W, Kadam S, Emerson BM, Bieker JJ.
 Mol Cell Biol. 2001 Apr;21(7):2413-22. [PMID: 11259590] 

Functional Description

 Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation (By similarity). 

Sequence Annotation

 ZN_FING 279 303 C2H2-type 1.
 ZN_FING 309 333 C2H2-type 2.
 ZN_FING 339 361 C2H2-type 3.
 MOD_RES 23 23 Phosphothreonine; by CK2 (Probable).
 MOD_RES 274 274 N6-acetyllysine (By similarity).
 MOD_RES 288 288 N6-acetyllysine (By similarity).
 CROSSLNK 54 54 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Acetylation; Activator; Complete proteome; Congenital dyserythropoietic anemia; Disease mutation; DNA-binding; Hereditary hemolytic anemia; Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; Ubl conjugation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 362 AA 

Protein Sequence

Gene Ontology

 GO:0000790; C:nuclear chromatin; IDA:BHF-UCL.
 GO:0000987; F:core promoter proximal region sequence-specific DNA binding; IDA:BHF-UCL.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006338; P:chromatin remodeling; IEA:Compara.
 GO:0035162; P:embryonic hemopoiesis; IEA:Compara.
 GO:0030218; P:erythrocyte differentiation; IMP:UniProtKB.
 GO:0043249; P:erythrocyte maturation; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 

Pfam

  

SMART

 SM00355; ZnF_C2H2 

PROSITE

 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 

PRINTS