CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017655
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Metal transporter CNNM3 
Protein Synonyms/Alias
 Ancient conserved domain-containing protein 3; Cyclin-M3 
Gene Name
 CNNM3 
Gene Synonyms/Alias
 ACDP3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
306DPYSDLSKGVLRCRTubiquitination[1, 2, 3, 4, 5, 6, 7, 8, 9]
462DYRDTVVKRKPASLMubiquitination[1, 2, 5, 6, 8, 9, 10]
464RDTVVKRKPASLMAPubiquitination[5]
475LMAPLKRKEEFSLFKubiquitination[6]
489KVSDDEYKVTISPQLubiquitination[7]
519SPLRISEKVLLHLLKubiquitination[1, 5, 6, 8]
526KVLLHLLKHPSVNQEubiquitination[1, 6, 8]
640LSDLQLIKVTRLQYLubiquitination[1, 5, 8]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [10] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
 Probable metal transporter (By similarity). 
Sequence Annotation
 DOMAIN 137 288 DUF21.
 DOMAIN 318 379 CBS 1.
 DOMAIN 386 452 CBS 2.
 MOD_RES 661 661 Phosphoserine.
 MOD_RES 700 700 Phosphoserine (By similarity).
 CARBOHYD 73 73 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; CBS domain; Cell membrane; Complete proteome; Glycoprotein; Ion transport; Membrane; Phosphoprotein; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 707 AA 
Protein Sequence
MAAAVAAAGR LGWLFAALCL GNAAGEAAPG PRVLGFCLEE DGAAGAGWVR GGAARDTPDA 60
TFLLRLFGPG FANSSWSWVA PEGAGCREEA ASPAGEWRAL LRLRLRAEAV RPHSALLAVR 120
VEPGGGAAEE AAPPWALGLG AAGLLALAAL ARGLQLSALA LAPAEVQVLR ESGSEAERAA 180
ARRLEPARRW AGCALGALLL LASLAQAALA VLLYRAAGQR AVPAVLGSAG LVFLVGEVVP 240
AAVSGRWTLA LAPRALGLSR LAVLLTLPVA LPVGQLLELA ARPGRLRERV LELARGGGDP 300
YSDLSKGVLR CRTVEDVLTP LEDCFMLDAS TVLDFGVLAS IMQSGHTRIP VYEEERSNIV 360
DMLYLKDLAF VDPEDCTPLS TITRFYNHPL HFVFNDTKLD AVLEEFKRGK SHLAIVQKVN 420
NEGEGDPFYE VLGLVTLEDV IEEIIRSEIL DESEDYRDTV VKRKPASLMA PLKRKEEFSL 480
FKVSDDEYKV TISPQLLLAT QRFLSREVDV FSPLRISEKV LLHLLKHPSV NQEVRFDESN 540
RLATHHYLYQ RSQPVDYFIL ILQGRVEVEI GKEGLKFENG AFTYYGVSAL TVPSSVHQSP 600
VSSLQPIRHD LQPDPGDGTH SSAYCPDYTV RALSDLQLIK VTRLQYLNAL LATRAQNLPQ 660
SPENTDLQVI PGSQTRLLGE KTTTAAGSSH SRPGVPVEGS PGRNPGV 707 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0006811; P:ion transport; IEA:UniProtKB-KW. 
Interpro
 IPR000644; Cysta_beta_synth_core.
 IPR002550; DUF21. 
Pfam
 PF01595; DUF21 
SMART
  
PROSITE
 PS51371; CBS 
PRINTS