UniProt Accession

 MESH1_HUMAN; Q8N4P3 

Genbank Protein ID

 AC068831; BC033794 

Genbank Nucleotide ID

 AAH33794.1 

Protein Name

 Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1 

Protein Synonyms/Alias

 HD domain-containing protein 3; Metazoan SpoT homolog 1; MESH1; Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase 

Gene Name

 HDDC3 

Gene Synonyms/Alias

 MESH1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
25	KHRQQRRKDPEGTPY	acetylation	[1]
97	TDDKTLPKLERKRLQ	acetylation	[1]
123	KLVKLADKLYNLRDL	acetylation	[1]
123	KLVKLADKLYNLRDL	ubiquitination	[2, 3, 4]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 ppGpp hydrolyzing enzyme involved in starvation response. 

Sequence Annotation

 DOMAIN 32 127 HD.
 ACT_SITE 65 65 Nucleophile (Probable).
 ACT_SITE 66 66 Nucleophile (Probable).
 METAL 35 35 Manganese.
 METAL 61 61 Manganese.
 METAL 62 62 Manganese.
 METAL 122 122 Manganese.
 MOD_RES 2 2 N-acetylglycine.
 MOD_RES 25 25 N6-acetyllysine.
 MOD_RES 97 97 N6-acetyllysine.
 MOD_RES 123 123 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Hydrolase; Manganese; Metal-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 179 AA 

Protein Sequence

Gene Ontology

 GO:0008893; F:guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity; IEA:EC.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008152; P:metabolic process; IEA:GOC. 

Interpro

 IPR026020; (p)ppGpp_Synthase.
 IPR003607; HD/PDEase_dom. 

Pfam

  

SMART

 SM00471; HDc 

PROSITE

  

PRINTS