CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-035932
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Glutamate--cysteine ligase catalytic subunit 
Protein Synonyms/Alias
  
Gene Name
 GCLC 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
28VLETLQEKGERTNPNubiquitination[1, 2, 3]
129FPDEAINKHPRFSTLacetylation[4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 154 AA 
Protein Sequence
MLVSFDHENK KVRLVLSGEK VLETLQEKGE RTNPNHPTLW RPEYGSYMIE GTPGQPYGGT 60
MSEFNTVEAN MRKRRKEATS ILEENQALCT ITSFPRLGCP GFTLPEVKPN PVEGGASKSL 120
FFPDEAINKH PRFSTLTRNI RHRRGEKVVI NVPI 154 
Gene Ontology
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0017109; C:glutamate-cysteine ligase complex; IEA:Compara.
 GO:0004357; F:glutamate-cysteine ligase activity; IEA:InterPro.
 GO:0006750; P:glutathione biosynthetic process; IEA:InterPro.
 GO:0019852; P:L-ascorbic acid metabolic process; IEA:Compara.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0031397; P:negative regulation of protein ubiquitination; IEA:Compara.
 GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; IEA:Compara.
 GO:0051900; P:regulation of mitochondrial depolarization; IEA:Compara.
 GO:0046685; P:response to arsenic-containing substance; IEA:Compara.
 GO:0051409; P:response to nitrosative stress; IEA:Compara.
 GO:0009410; P:response to xenobiotic stimulus; IEA:Compara. 
Interpro
 IPR004308; GCS. 
Pfam
  
SMART
  
PROSITE
  
PRINTS