CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020261
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kinesin-like protein KIFC1 
Protein Synonyms/Alias
 Kinesin-like protein 2; Kinesin-related protein HSET 
Gene Name
 KIFC1 
Gene Synonyms/Alias
 HSET; KNSL2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
23ELKRPLIKAPSQLPLubiquitination[1]
308HNQLQELKGNIRVFCubiquitination[2, 3, 4, 5, 6, 7]
511YVPVSCEKEVDALLHubiquitination[2, 4, 6, 7]
619HVPYRNSKLTYLLQNubiquitination[1, 2, 4, 5, 6, 7]
659NSLRFASKVNQCVIGubiquitination[1, 3, 4, 7]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles. 
Sequence Annotation
 DOMAIN 307 594 Kinesin-motor.
 NP_BIND 410 417 ATP (By similarity).
 MOD_RES 31 31 Phosphoserine.
 MOD_RES 33 33 Phosphoserine (By similarity).  
Keyword
 3D-structure; ATP-binding; Cell cycle; Cell division; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Endosome; Microtubule; Mitosis; Motor protein; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 673 AA 
Protein Sequence
MDPQRSPLLE VKGNIELKRP LIKAPSQLPL SGSRLKRRPD QMEDGLEPEK KRTRGLGATT 60
KITTSHPRVP SLTTVPQTQG QTTAQKVSKK TGPRCSTAIA TGLKNQKPVP AVPVQKSGTS 120
GVPPMAGGKK PSKRPAWDLK GQLCDLNAEL KRCRERTQTL DQENQQLQDQ LRDAQQQVKA 180
LGTERTTLEG HLAKVQAQAE QGQQELKNLR ACVLELEERL STQEGLVQEL QKKQVELQEE 240
RRGLMSQLEE KERRLQTSEA ALSSSQAEVA SLRQETVAQA ALLTEREERL HGLEMERRRL 300
HNQLQELKGN IRVFCRVRPV LPGEPTPPPG LLLFPSGPGG PSDPPTRLSL SRSDERRGTL 360
SGAPAPPTRH DFSFDRVFPP GSGQDEVFEE IAMLVQSALD GYPVCIFAYG QTGSGKTFTM 420
EGGPGGDPQL EGLIPRALRH LFSVAQELSG QGWTYSFVAS YVEIYNETVR DLLATGTRKG 480
QGGECEIRRA GPGSEELTVT NARYVPVSCE KEVDALLHLA RQNRAVARTA QNERSSRSHS 540
VFQLQISGEH SSRGLQCGAP LSLVDLAGSE RLDPGLALGP GERERLRETQ AINSSLSTLG 600
LVIMALSNKE SHVPYRNSKL TYLLQNSLGG SAKMLMFVNI SPLEENVSES LNSLRFASKV 660
NQCVIGTAQA NRK 673 
Gene Ontology
 GO:0005769; C:early endosome; IEA:UniProtKB-SubCell.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005875; C:microtubule associated complex; NAS:UniProtKB.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; NAS:UniProtKB.
 GO:0003777; F:microtubule motor activity; NAS:UniProtKB.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007018; P:microtubule-based movement; TAS:Reactome.
 GO:0000070; P:mitotic sister chromatid segregation; NAS:UniProtKB. 
Interpro
 IPR019821; Kinesin_motor_CS.
 IPR001752; Kinesin_motor_dom.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00225; Kinesin 
SMART
 SM00129; KISc 
PROSITE
 PS00411; KINESIN_MOTOR_DOMAIN1
 PS50067; KINESIN_MOTOR_DOMAIN2 
PRINTS
 PR00380; KINESINHEAVY.