Tag | Content |
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CPLM ID | CPLM-005559 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Surfactin synthase subunit 1 |
Protein Synonyms/Alias | |
Gene Name | srfAA |
Gene Synonyms/Alias | srfA; srfA1; BSU03480 |
Created Date | July 27, 2013 |
Organism | Bacillus subtilis (strain 168) |
NCBI Taxa ID | 224308 |
Lysine Modification | Position | Peptide | Type | References |
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1234 | RQAAEGYKKDQAYWK | acetylation | [1] |
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Reference | [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis. Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG. Proteomics. 2013 May;13(10-11):1726-36. [ PMID: 23468065] |
Functional Description | This protein is a multifunctional enzyme able to activate and polymerize the amino acids Leu, Glu, Asp and Val. Activation sites for these AA consist of individual domains. |
Sequence Annotation | DOMAIN 976 1043 Acyl carrier 1. DOMAIN 2015 2082 Acyl carrier 2. DOMAIN 3043 3109 Acyl carrier 3. REGION ? 1047 Domain 1 (glutamate-activating). REGION ? 2086 Domain 2 (leucine-activating). REGION ? 3114 Domain 3 (D-leucine-activating). MOD_RES 1006 1006 O-(pantetheine 4'-phosphoryl)serine MOD_RES 2045 2045 O-(pantetheine 4'-phosphoryl)serine MOD_RES 3073 3073 O-(pantetheine 4'-phosphoryl)serine |
Keyword | Antibiotic biosynthesis; Complete proteome; Ligase; Multifunctional enzyme; Phosphopantetheine; Phosphoprotein; Reference proteome; Repeat; Sporulation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 3587 AA |
Protein Sequence | MEITFYPLTD AQKRIWYTEK FYPHTSISNL AGIGKLVSAD AIDYVLVEQA IQEFIRRNDA 60 MRLRLRLDEN GEPVQYISEY RPVDIKHTDT TEDPNAIEFI SQWSREETKK PLPLYDCDLF 120 RFSLFTIKEN EVWFYANVHH VISDGISMNI LGNAIMHIYL ELASGSETKE GISHSFIDHV 180 LSEQEYAQSK RFEKDKAFWN KQFESVPELV SLKRNASAGG SLDAERFSKD VPEALHQQIL 240 SFCEANKVSV LSVFQSLLAA YLYRVSGQND VVTGTFMGNR TNAKEKQMLG MFVSTVPLRT 300 NIDGGQAFSE FVKDRMKDLM KTLRHQKYPY NLLINDLRET KSSLTKLFTV SLEYQVMQWQ 360 KEEDLAFLTE PIFSGSGLND VSIHVKDRWD TGKLTIDFDY RTDLFSREEI NMICERMITM 420 LENALTHPEH TIDELTLISD AEKEKLLARA GGKSVSYRKD MTIPELFQEK AELLSDHPAV 480 VFEDRTLSYR TLHEQSARIA NVLKQKGVGP DSPVAVLIER SERMITAIMG ILKAGGAYVP 540 IDPGFPAERI QYILEDCGAD FILTESKVAA PEADAELIDL DQAIEEGAEE SLNADVNARN 600 LAYIIYTSGT TGRPKGVMIE HRQVHHLVES LQQTIYQSGS QTLRMALLAP FHFDASVKQI 660 FASLLLGQTL YIVPKKTVTN GAALTAYYRK NSIEATDGTP AHLQMLAAAG DFEGLKLKHM 720 LIGGEGLSSV VADKLLKLFK EAGTAPRLTN VYGPTETCVD ASVHPVIPEN AVQSAYVPIG 780 KALGNNRLYI LDQKGRLQPE GVAGELYIAG DGVGRGYLHL PELTEEKFLQ DPFVPGDRMY 840 RTGDVVRWLP DGTIEYLGRE DDQVKVRGYR IELGEIEAVI QQAPDVAKAV VLARPDEQGN 900 LEVCAYVVQK PGSEFAPAGL REHAARQLPD YMVPAYFTEV TEIPLTPSGK VDRRKLFALE 960 VKAVSGTAYT APRNETEKAI AAIWQDVLNV EKAGIFDNFF ETGGHSLKAM TLLTKIHKET 1020 GIEIPLQFLF EHPTITALAE EADHRESKAF AVIEPAEKQE HYPLSLAQQR TYIVSQFEDA 1080 GVGYNMPAAA ILEGPLDIQK LERAFQGLIR RHESLRTSFV LENSTPRQKI HDSVDFNIEM 1140 IERGGRSDEA IMASFVRTFD LAKAPLFRIG LLGLEENRHM LLFDMHHLIS DGVSIGIMLE 1200 ELARIYKGEQ LPDLRLQYKD YAVWQSRQAA EGYKKDQAYW KEVFAGELPV LQLLSDYPRP 1260 PVQSFEGDRV SIKLDAGVKD RLNRLAEQNG ATLYMVMLSA YYTLLSKYTG QDDIIVGTPS 1320 AGRNHSDTEG IIGMFVNTLA IRSEVKQNET FTQLISRVRK RVLDAFSHQD YPFEWLVEDL 1380 NIPRDVSRHP LFDTMFSLQN ATEGIPAVGD LSLSVQETNF KIAKFDLTVQ ARETDEGIEI 1440 DVDYSTKLFK QSTADRLLTH FARLLEDAAA DPEKPISEYK LLSEEEAASQ IQQFNPGRTP 1500 YPKDKTIVQL FEEQAANTPD HTALQYEGES LTYRELNERA NRLARGILSL GAGEGRTAAV 1560 LCERSMDMIV SILAVLKSGS AYVPIDPEHP IQRMQHFFRD SGAKVLLTQR KLKALAEEAE 1620 FKGVIVLADE EESYHADARN LALPLDSAAM ANLTYTSGTT GTPKGNIVTH ANILRTVKET 1680 NYLSITEQDT ILGLSNYVFD AFMFDMFGSL LNGAKLVLIP KETVLDMARL SRVIERENIS 1740 ILMITTALFH LLVDLNPACL STLRKIMFGG ERASVEHVRK ALQTVGKGKL LHMYGPSEST 1800 VFATYHPVDE LEEHTLSVPI GKPVSNTEVY ILDRTGHVQP AGIAGELCVS GEGLVKGYYN 1860 RPELTEEKFV PHPFTSGERM YKTGDLARWL PNGDIEFIGR IDHQVKIRGQ RIELGEIEHQ 1920 LQTHDRVQES VVLAVDQGAG DKLLCAYYVG EGDISSQEMR EHAAKDLPAY MVPAVFIQMD 1980 ELPLTGNGKI DRRALPIPDA NVSRGVSYVA PRNGTEQKVA DIWAQVLQAE QVGAYDHFFD 2040 IGGHSLAGMK MLALVHQELG VELSLKDLFQ SPTVEGLAQV IASAEKGTAA SISPAEKQDT 2100 YPVSSPQKRM YVLQQLEDAQ TSYNMPAVLR LTGELDVERL NSVMQQLMQR HEALRTTFEI 2160 KDGETVQRIW EEAECEIAYF EAPEEETERI VSEFIKPFKI DQLPLFRIGL IKHSDTEHVL 2220 LFDMHHIISD GASVGVLIEE LSKLYDGETL EPLRIQYKDY AVWQQQFIQS ELYKKQEEHW 2280 LKELDGELPV LTLPTDYSRP AVQTFEGDRI AFSLEAGKAD ALRRLAKETD STLYMVLLAS 2340 YSAFLSKISG QDDIIVGSPV AGRSQADVSR VIGMFVNTLA LRTYPKGEKT FADYLNEVKE 2400 TALSAFDAQD YPLEDLIGNV QVQRDTSRNP LFDAVFSMQN ANIKDLTMKG IQLEPHPFER 2460 KTAKFDLTLT ADETDGGLTF VLEYNTALFK QETIERWKQY WMELLDAVTG NPNQPLSSLS 2520 LVTETEKQAL LEAWKGKALP VPTDKTVHQL FEETAQRHKD RPAVTYNGQS WTYGELNAKA 2580 NRLARILMDC GISPDDRVGV LTKPSLEMSA AVLGVLKAGA AFVPIDPDYP DQRIEYILQD 2640 SGAKLLLKQE GISVPDSYTG DVILLDGSRT ILSLPLDEND EENPETAVTA ENLAYMIYTS 2700 GTTGQPKGVM VEHHALVNLC FWHHDAFSMT AEDRSAKYAG FGFDASIWEM FPTWTIGAEL 2760 HVIEEAIRLD IVRLNDYFET NGVTITFLPT QLAEQFMELE NTSLRVLLTG GDKLKRAVKK 2820 PYTLVNNYGP TENTVVATSA EIHPEEGSLS IGRAIANTRV YILGEGNQVQ PEGVAGELCV 2880 AGRGLARGYL NREDETAKRF VADPFVPGER MYRTGDLVKW TGGGIEYIGR IDQQVKVRGY 2940 RIELSEIEVQ LAQLSEVQDA AVTAVKDKGG NTAIAAYVTP ESADIEALKS ALKETLPDYM 3000 IPAFWVTLNE LPVTANGKVD RKALPEPDIE AGSGEYKAPT TDMEELLAGI WQDVLGMSEV 3060 GVTDNFFSLG GDSIKGIQMA SRLNQHGWKL EMKDLFQHPT IEELTQYVER AEGKQADQGP 3120 VEGEVILTPI QRWFFEKNFT NKHHWNQSVM LHAKKGFDPE RVEKTLQALI EHHDALRMVY 3180 REGQEDVIQY NRGLEAASAQ LEVIQIEGQA ADYEDRIERE AERLQSSIDL QEGGLLKAGL 3240 FQAEDGDHLL LAIHHLVVDG VSWRILLEDF AAVYTQLEQG NEPVLPQKTH SFAEYAERLQ 3300 DFANSKAFLK EKEYWRQLEE QAVAAKLPKD RESGDQRMKH TKTIEFSLTA EETEQLTTKV 3360 HEAYHTEMND ILLTAFGLAM KEWTGQDRVS VHLEGHGREE IIEDLTISRT VGWFTSMYPM 3420 VLDMKHADDL GYQLKQMKED IRHVPNKGVG YGILRYLTAP EHKEDVAFSI QPDVSFNYLG 3480 QFDEMSDAGL FTRSELPSGQ SLSPETEKPN ALDVVGYIEN GKLTMSLAYH SLEFHEKTVQ 3540 TFSDSFKAHL LRIIEHCLSQ DGTELTPSDL GDDDLTLDEL DKLMEIF 3587 |
Gene Ontology | GO:0016874; F:ligase activity; IEA:UniProtKB-KW. GO:0031177; F:phosphopantetheine binding; IEA:InterPro. GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW. GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |