CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004433
UniProt Accession
Genbank Protein ID
 J05056 
Genbank Nucleotide ID
Protein Name
 Oxysterol-binding protein 1 
Protein Synonyms/Alias
  
Gene Name
 OSBP 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Oryctolagus cuniculus (Rabbit) 
NCBI Taxa ID
 9986 
Lysine Modification
Position
Peptide
Type
References
431IMKNCIGKELSKIPMubiquitination[1]
435CIGKELSKIPMPVNFubiquitination[1]
606EIDIVNHKTGDKCNLubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Binds cholesterol and a range of oxysterols. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylate ERK1/2, whereas 25- hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing ABCA1 stability. 
Sequence Annotation
 DOMAIN 90 183 PH.
 REGION 408 459 Sterol binding.
 MOD_RES 105 105 N6-acetyllysine (By similarity).
 MOD_RES 192 192 Phosphoserine (By similarity).
 MOD_RES 195 195 Phosphoserine (By similarity).
 MOD_RES 200 200 Phosphoserine (By similarity).
 MOD_RES 240 240 Phosphoserine (By similarity).
 MOD_RES 353 353 Phosphoserine (By similarity).
 MOD_RES 379 379 Phosphothreonine (By similarity).
 MOD_RES 381 381 Phosphoserine (By similarity).
 MOD_RES 384 384 Phosphoserine (By similarity).
 MOD_RES 391 391 Phosphoserine (By similarity).  
Keyword
 Acetylation; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Golgi apparatus; Lipid transport; Lipid-binding; Membrane; Phosphoprotein; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 809 AA 
Protein Sequence
MAATELRGVV GPGPAAIAAP GGGGAGPPVV GGGGGGRGDA GPGSGAASGT VAAAAAGGQG 60
PGAGGVAAAA GPAPTPPAGG SGSSGTGGSG SAREGWLFKW TNYIKGYQRR WFVLSNGLLS 120
YYRSKAEMRH TCRGTINLAT ANITVEDSCN FIISNGGAQT YHLKASSEVE RQRWVTALEL 180
AKAKAVKMLA ESDESGDEES VSQTDKTELQ NTLRTLSSKV EDLSTCNDLI AKHGTALQRS 240
LSELESLKLP AESNEKIKQV NERATLFRIT SNAMINACRD FLVLAQTHSK KWQKSLQYER 300
DQRIRLEETL EQLAKQHNHL ERAFRGATVL PAHTSGSAGS GKDQCCSGKG DMSDEDDENE 360
FFDAPEIITM PENLGHKRTG SNISGASSDI SLDEQYKHQL EETKKEKRTR IPYKPNYSLN 420
LWSIMKNCIG KELSKIPMPV NFNEPLSMLQ RLTEDLEYHE LLDRAAKCEN SLEQLCYVAA 480
FTVSSYSTTV FRTSKPFNPL LGETFELDRL EENGYRSLCE QVSHHPPAAA HHAESKNGWT 540
LRQEIKITSK FRGKYLSIMP LGTIHCIFHA TGHHYTWKKV TTTVHNIIVG KLWIDQSGEI 600
DIVNHKTGDK CNLKFVPYSY FSRDVARKVT GEVTDPSGKV HFALLGTWDE KMDCFKVQPV 660
SGENGGDARQ RGHEAEESRV MLWKRNPLPK NAENMYYFSE LALTLNAWEG GTAPTDSRLR 720
PDQRLMENGR WDEANAEKQR LEEKQRLSRK KREAEAMKAT EDGTPYDPYK ALWFERKKDP 780
VTKELTHIYR GEYWECKEKQ DWNSCPDIF 809 
Gene Ontology
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0015485; F:cholesterol binding; IDA:BHF-UCL.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0006869; P:lipid transport; IEA:UniProtKB-KW. 
Interpro
 IPR000648; Oxysterol-bd.
 IPR018494; Oxysterol-bd_CS.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology. 
Pfam
 PF01237; Oxysterol_BP
 PF00169; PH 
SMART
 SM00233; PH 
PROSITE
 PS01013; OSBP
 PS50003; PH_DOMAIN 
PRINTS