CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006887
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 3 subunit A 
Protein Synonyms/Alias
 eIF3a; Eukaryotic translation initiation factor 3 110 kDa subunit homolog; eIF3 p110; Translation initiation factor eIF3, p110 subunit homolog 
Gene Name
 RPG1 
Gene Synonyms/Alias
 TIF32; YBR079C; YBR0734 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
69LKKGKLLKDGLHQYKacetylation[1]
95SVGAVARKFIDLVESacetylation[1]
192KTMHFCLKYQRKNEFmethylation[2]
268EDVFHLMKISKRAPKacetylation[1]
308TAWKKFYKLYSTNPRacetylation[1]
360RLLNLDAKPTRKEMLubiquitination[3]
599DDLEKISKIVDERVKacetylation[1]
698SLDLDTIKQVIIAEVubiquitination[4]
744LLQKEVDKLQETDTAacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Identification of arginine- and lysine-methylation in the proteome of Saccharomyces cerevisiae and its functional implications.
 Pang CN, Gasteiger E, Wilkins MR.
 BMC Genomics. 2010 Feb 5;11:92. [PMID: 20137074]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [4] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. 
Sequence Annotation
 DOMAIN 382 493 PCI.
 MOD_RES 508 508 Phosphoserine.
 MOD_RES 691 691 Phosphoserine.
 MOD_RES 935 935 Phosphothreonine.  
Keyword
 Coiled coil; Complete proteome; Cytoplasm; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 964 AA 
Protein Sequence
MAPPPFRPEN AIKRADELIS VGEKQAALQS LHDFITARRI RWATPSTVEP VVFKFLEIGV 60
ELKKGKLLKD GLHQYKKLIQ GSTEGLVSVG AVARKFIDLV ESKIASEQTR ADELQKQEID 120
DDLEGGVTPE NLLISVYESD QSVAGFNDEA ITSWLRFTWE SYRAVLDLLR NNALLEITYS 180
GVVKKTMHFC LKYQRKNEFK RLAEMLRQHL DAANYQQSKS GNNLVDLSDA DTLQRYLDQR 240
FQQVDVSVKL ELWHEAYRSI EDVFHLMKIS KRAPKPSTLA NYYENLVKVF FVSGDPLLHT 300
TAWKKFYKLY STNPRATEEE FKTYSSTIFL SAISTQLDEI PSIGYDPHLR MYRLLNLDAK 360
PTRKEMLQSI IEDESIYGKV DEELKELYDI IEVNFDVDTV KQQLENLLVK LSSKTYFSQY 420
IAPLRDVIMR RVFVAASQKF TTVSQSELYK LATLPAPLDL SAWDIEKSLL QAAVEDYVSI 480
TIDHESAKVT FAKDPFDIFA STASKEVSEE ENTEPEVQEE KEETDEALGP QETEDGEEKE 540
EESDPVIIRN SYIHNKLLEL SNVLHDVDSF NNASYMEKVR IARETLIKKN KDDLEKISKI 600
VDERVKRSQE QKQKHMEHAA LHAEQDAEVR QQRILEEKAA IEAKLEEEAH RRLIEKKKRE 660
FEAIKEREIT KMITEVNAKG HVYIDPNEAK SLDLDTIKQV IIAEVSKNKS ELESRMEYAM 720
KKLDHTERAL RKVELPLLQK EVDKLQETDT ANYEAMKKKI VDAAKAEYEA RMADRKNLVM 780
VYDDYLKFKE HVSGTKESEL AAIRNQKKAE LEAAKKARIE EVRKRRYEEA IARRKEEIAN 840
AERQKRAQEL AEATRKQREI EEAAAKKSTP YSFRAGNREP PSTPSTLPKA TVSPDKAKLD 900
MIAQKQREME EAIEQRLAGR TAGGSSPATP ATPATPATPT PSSGPKKMTM AEKLRAKRLA 960
KGGR 964 
Gene Ontology
 GO:0010494; C:cytoplasmic stress granule; IDA:SGD.
 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; IPI:SGD.
 GO:0000131; C:incipient cellular bud site; IDA:SGD.
 GO:0003743; F:translation initiation factor activity; IDA:SGD.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006446; P:regulation of translational initiation; IEA:HAMAP.
 GO:0002188; P:translation reinitiation; IMP:SGD. 
Interpro
 IPR027512; EIF3A.
 IPR000717; PCI_dom. 
Pfam
  
SMART
 SM00088; PINT 
PROSITE
  
PRINTS