CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016144
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutamine-dependent NAD(+) synthetase 
Protein Synonyms/Alias
 NAD(+) synthase [glutamine-hydrolyzing]; NAD(+) synthetase 
Gene Name
 Nadsyn1 
Gene Synonyms/Alias
 Qns1 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
28GNFQRILKSIQIAKGubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
  
Sequence Annotation
 DOMAIN 5 299 CN hydrolase.
 NP_BIND 355 362 ATP (By similarity).
 REGION 325 706 Ligase (By similarity).
 ACT_SITE 357 357 By similarity.  
Keyword
 ATP-binding; Complete proteome; Ligase; NAD; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 725 AA 
Protein Sequence
MGRKVTVATC ALNQWALDFE GNFQRILKSI QIAKGKGARY RLGPELEICG YGCWDHYHES 60
DTLLHSLQVL AALLDAPATQ DIICDVGMPI MHRNVRYNCL VIFLNRKILL IRPKMALANE 120
GNYRELRWFT PWARSRQTEE YVLPRMLQDL TKQETVPFGD VVLATQDTCI GSEICEELWT 180
PCSPHVNMGL DGVEIITNAS GSHHVLRKAH TRVDLVTMAT SKNGGIYLLA NQKGCDGHLL 240
YYDGCAMIAM NGSIFAQGTQ FSLDDVEVLT ATLDLEDVRS YRAKISSRNL EATRVNPYPR 300
VTVDFALSVS EDLLEPVSEP VEWTYHRPEE EISLGPACWL WDFLRRNNQA GFFLPLSGGV 360
DSAASACVVY SMCCLVCEAV KSGNQQVLTD VQNLVDESSY TPQDPRELCG RLLTTCYMAS 420
ENSSQETHNR ATELAQQIGS YHISLNIDPA VKAILGIFSL VTGKFPRFSA HGGSSRENLA 480
LQNVQARIRM VLAYLFAQLS LWSRGARGSL LVLGSANVDE SLLGYLTKYD CSSADINPIG 540
GISKTDLRAF VQLCAERFQL PVLQAILSAP ATAELEPLAD GQVSQMDEED MGMTYTELSI 600
FGRLRKVAKA GPYSMFCKLL NMWKDSCTPR QVAEKVKRFF SKYSINRHKM TTLTPAYHAE 660
NYSPDDNRFD LRPFLYNTRW PWQFLCIDNQ VVQLERKTSQ TLEEQIQEHF KEPSPIWKQL 720
LPKDP 725 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016810; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds; IEA:InterPro.
 GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; IEA:EC.
 GO:0009435; P:NAD biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR003010; C-N_Hydrolase.
 IPR014445; Gln-dep_NAD_synthase.
 IPR022310; NAD/GMP_synthase.
 IPR003694; NAD_synthase.
 IPR014729; Rossmann-like_a/b/a_fold. 
Pfam
 PF00795; CN_hydrolase
 PF02540; NAD_synthase 
SMART
  
PROSITE
 PS50263; CN_HYDROLASE 
PRINTS