UniProt Accession

 AT1A1_RAT; P06685; Q64609 

Genbank Protein ID

 M14511; X05882; M28647; BC061968; M11733; X53233; X53234 

Genbank Nucleotide ID

 AAA40775.1; CAA29306.1; AAA41671.1; AAH61968.1; AAA40783.1; CAA37325.1; CAA37326.1 

Protein Name

 Sodium/potassium-transporting ATPase subunit alpha-1 

Protein Synonyms/Alias

 Na(+)/K(+) ATPase alpha-1 subunit; Sodium pump subunit alpha-1 

Gene Name

 Atp1a1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
9	GKGVGRDKYEPAAVS	ubiquitination	[1]
36	ERDMDELKKEVSMDD	acetylation	[2]
36	ERDMDELKKEVSMDD	ubiquitination	[1]
37	RDMDELKKEVSMDDH	ubiquitination	[1]
45	EVSMDDHKLSLDELH	acetylation	[2]
45	EVSMDDHKLSLDELH	ubiquitination	[1]
156	YQEAKSSKIMESFKN	acetylation	[2]
156	YQEAKSSKIMESFKN	ubiquitination	[1]
162	SKIMESFKNMVPQQA	acetylation	[2]
444	QENLPILKRAVAGDA	acetylation	[2]
444	QENLPILKRAVAGDA	ubiquitination	[1]
473	SVMEMREKYTKIVEI	acetylation	[2]
476	EMREKYTKIVEIPFN	acetylation	[2]
487	IPFNSTNKYQLSIHK	acetylation	[2]
494	KYQLSIHKNPNASEP	acetylation	[2]
494	KYQLSIHKNPNASEP	ubiquitination	[1]
502	NPNASEPKHLLVMKG	acetylation	[2]
629	ITAKAIAKGVGIISE	ubiquitination	[1]
671	VVHGSDLKDMTSEEL	acetylation	[2]
726	VNDSPALKKADIGVA	ubiquitination	[1]
727	NDSPALKKADIGVAM	ubiquitination	[1]
843	PRNPKTDKLVNERLI	acetylation	[2]
1019	RPGGWVEKETYY***	acetylation	[2]

Reference

 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. 

Sequence Annotation

 REGION 82 84 Phosphoinositide-3 kinase binding.
 ACT_SITE 376 376 4-aspartylphosphate intermediate (By
 METAL 717 717 Magnesium (By similarity).
 METAL 721 721 Magnesium (By similarity).
 BINDING 487 487 ATP.
 MOD_RES 10 10 Phosphotyrosine.
 MOD_RES 16 16 Phosphoserine; by PKC.
 MOD_RES 23 23 Phosphoserine; by PKC.
 MOD_RES 47 47 Phosphoserine (By similarity).
 MOD_RES 217 217 Phosphoserine (By similarity).
 MOD_RES 219 219 Phosphothreonine (By similarity).
 MOD_RES 228 228 Phosphoserine (By similarity).
 MOD_RES 260 260 Phosphotyrosine (By similarity).
 MOD_RES 452 452 Phosphoserine (By similarity).
 MOD_RES 542 542 Phosphotyrosine (By similarity).
 MOD_RES 943 943 Phosphoserine; by PKA.  

Keyword

 3D-structure; ATP-binding; Cell membrane; Complete proteome; Direct protein sequencing; Hydrolase; Ion transport; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Potassium; Potassium transport; Reference proteome; Sodium; Sodium transport; Sodium/potassium transport; Transmembrane; Transmembrane helix; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1023 AA 

Protein Sequence

Gene Ontology

 GO:0016323; C:basolateral plasma membrane; IEA:Compara.
 GO:0005901; C:caveola; IDA:RGD.
 GO:0005783; C:endoplasmic reticulum; IDA:BHF-UCL.
 GO:0005768; C:endosome; IDA:RGD.
 GO:0005794; C:Golgi apparatus; IDA:BHF-UCL.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0005890; C:sodium:potassium-exchanging ATPase complex; IDA:RGD.
 GO:0030315; C:T-tubule; IEA:Compara.
 GO:0003869; F:4-nitrophenylphosphatase activity; IEA:Compara.
 GO:0043531; F:ADP binding; IDA:RGD.
 GO:0005524; F:ATP binding; IDA:RGD.
 GO:0051087; F:chaperone binding; IDA:BHF-UCL.
 GO:0030955; F:potassium ion binding; IDA:RGD.
 GO:0031402; F:sodium ion binding; IDA:RGD.
 GO:0005391; F:sodium:potassium-exchanging ATPase activity; IDA:UniProtKB.
 GO:0006754; P:ATP biosynthetic process; IEA:InterPro.
 GO:0071260; P:cellular response to mechanical stimulus; IDA:RGD.
 GO:0015988; P:energy coupled proton transmembrane transport, against electrochemical gradient; TAS:RGD.
 GO:0060081; P:membrane hyperpolarization; IMP:RGD.
 GO:0031947; P:negative regulation of glucocorticoid biosynthetic process; IEA:Compara.
 GO:0045822; P:negative regulation of heart contraction; IEA:Compara.
 GO:0045823; P:positive regulation of heart contraction; IEA:Compara.
 GO:0045989; P:positive regulation of striated muscle contraction; IEA:Compara.
 GO:0010107; P:potassium ion import; IDA:RGD.
 GO:0008217; P:regulation of blood pressure; IEA:Compara.
 GO:0086004; P:regulation of cardiac muscle cell contraction; IMP:RGD.
 GO:0002028; P:regulation of sodium ion transport; IDA:UniProtKB.
 GO:0002026; P:regulation of the force of heart contraction; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara. 

Interpro

 IPR006068; ATPase_P-typ_cation-transptr_C.
 IPR004014; ATPase_P-typ_cation-transptr_N.
 IPR023299; ATPase_P-typ_cyto_domN.
 IPR005775; ATPase_P-typ_Na/K_IIC.
 IPR018303; ATPase_P-typ_P_site.
 IPR023298; ATPase_P-typ_TM_dom.
 IPR008250; ATPase_P-typ_transduc_dom_A.
 IPR001757; Cation_transp_P_typ_ATPase.
 IPR023214; HAD-like_dom. 

Pfam

 PF00689; Cation_ATPase_C
 PF00690; Cation_ATPase_N
 PF00122; E1-E2_ATPase
 PF00702; Hydrolase 

SMART

 SM00831; Cation_ATPase_N 

PROSITE

 PS00154; ATPASE_E1_E2 

PRINTS

 PR00119; CATATPASE.