CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029689
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Alpha-enolase 
Protein Synonyms/Alias
  
Gene Name
 Eno1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
28EVDLYTAKGLFRAAVacetylation[1]
28EVDLYTAKGLFRAAVubiquitination[2]
54LELRDNDKTRFMGKGacetylation[3]
54LELRDNDKTRFMGKGubiquitination[2]
81APALCTQKVNVVEQEubiquitination[2]
89VNVVEQEKIDKLMIEacetylation[3, 4, 5, 6, 7]
89VNVVEQEKIDKLMIEsuccinylation[6]
89VNVVEQEKIDKLMIEubiquitination[2]
92VEQEKIDKLMIEMDGacetylation[1, 4, 6]
92VEQEKIDKLMIEMDGubiquitination[2]
103EMDGTENKSKFGANAacetylation[6]
103EMDGTENKSKFGANAsuccinylation[6]
103EMDGTENKSKFGANAubiquitination[2]
105DGTENKSKFGANAILacetylation[1]
126CKAGAVEKGVPLYRHacetylation[1, 3, 6]
193AEVYHNLKNVIKEKYacetylation[3, 4, 6]
193AEVYHNLKNVIKEKYphosphoglycerylation[8]
193AEVYHNLKNVIKEKYsuccinylation[6]
193AEVYHNLKNVIKEKYubiquitination[2]
199LKNVIKEKYGKDATNacetylation[1]
202VIKEKYGKDATNVGDacetylation[1, 3, 4, 6, 7]
202VIKEKYGKDATNVGDsuccinylation[6]
202VIKEKYGKDATNVGDubiquitination[2]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [5] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [6] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [7] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [8] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 221 AA 
Protein Sequence
MSILRIHARE IFDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DNDKTRFMGK 60
GVSRPVKYVN EFLAPALCTQ KVNVVEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK 120
AGAVEKGVPL YRHIADLAGN PEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGASSFRE 180
AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN K 221 
Gene Ontology
 GO:0000015; C:phosphopyruvate hydratase complex; IEA:InterPro.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0004634; F:phosphopyruvate hydratase activity; IEA:InterPro.
 GO:0006096; P:glycolysis; IEA:InterPro. 
Interpro
 IPR000941; Enolase.
 IPR020810; Enolase_C.
 IPR020811; Enolase_N. 
Pfam
 PF00113; Enolase_C
 PF03952; Enolase_N 
SMART
  
PROSITE
  
PRINTS
 PR00148; ENOLASE.