UniProt Accession

 SYDC_MOUSE; Q922B2; Q3U987 

Genbank Protein ID

 AK151027; AK151897; AK152304; AK152562; AK153256; AK153299; AK169716; BC008638 

Genbank Nucleotide ID

 BAE30045.1; BAE30780.1; BAE31110.1; BAE31315.1; BAE31847.1; BAE31881.1; BAE41327.1; AAH08638.1 

Protein Name

 Aspartate--tRNA ligase, cytoplasmic 

Protein Synonyms/Alias

 Aspartyl-tRNA synthetase; AspRS 

Gene Name

 Dars 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
74	HTSRAKGKQCFLVLR	ubiquitination	[1]
99	AVGDHASKQMVKFAA	ubiquitination	[1]
103	HASKQMVKFAANINK	acetylation	[2]
103	HASKQMVKFAANINK	ubiquitination	[1]
110	KFAANINKESIIDVE	ubiquitination	[1]
241	VFTVSYFKNNAYLAQ	ubiquitination	[1]
330	TEIQTVSKQFPCEPF	ubiquitination	[1]
383	LGRLVKEKYDTDFYV	ubiquitination	[1]
411	MPDPRNPKQSNSYDM	ubiquitination	[1]
451	HHGIDLEKIKAYIDS	acetylation	[3]
451	HHGIDLEKIKAYIDS	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

 Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. 

Sequence Annotation

 MOD_RES 74 74 N6-acetyllysine (By similarity).
 MOD_RES 249 249 Phosphoserine (By similarity).
 MOD_RES 374 374 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 501 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004815; F:aspartate-tRNA ligase activity; IEA:EC.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0006422; P:aspartyl-tRNA aminoacylation; IEA:InterPro. 

Interpro

 IPR004364; aa-tRNA-synt_II.
 IPR018150; aa-tRNA-synt_II-like.
 IPR006195; aa-tRNA-synth_II.
 IPR004523; Asp-tRNA_synthase.
 IPR002312; Asp/Asn-tRNA-synth_IIb.
 IPR012340; NA-bd_OB-fold.
 IPR004365; NA-bd_OB_tRNA-helicase. 

Pfam

 PF00152; tRNA-synt_2
 PF01336; tRNA_anti 

SMART

  

PROSITE

 PS50862; AA_TRNA_LIGASE_II 

PRINTS

 PR01042; TRNASYNTHASP.