CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007959
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 
Protein Synonyms/Alias
 D-fructose-6-phosphate amidotransferase 1; Glutamine:fructose-6-phosphate amidotransferase 1; GFAT 1; GFAT1; Hexosephosphate aminotransferase 1 
Gene Name
 Gfpt1 
Gene Synonyms/Alias
 Gfpt 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
25EILETLIKGLQRLEYubiquitination[1]
48GLDGGNDKDWEANACubiquitination[1]
377GGLKDHIKEIQRCRRacetylation[2]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. 
Sequence Annotation
 DOMAIN 2 303 Glutamine amidotransferase type-2.
 DOMAIN 375 514 SIS 1.
 DOMAIN 546 687 SIS 2.
 REGION 311 678 Isomerase (By similarity).
 REGION 392 393 Substrate-binding (By similarity).
 REGION 437 439 Substrate-binding (By similarity).
 ACT_SITE 2 2 For GATase activity (By similarity).
 BINDING 442 442 Substrate (By similarity).
 BINDING 582 582 Substrate (By similarity).
 MOD_RES 259 259 Phosphoserine.  
Keyword
 Alternative splicing; Aminotransferase; Complete proteome; Glutamine amidotransferase; Phosphoprotein; Reference proteome; Repeat; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 697 AA 
Protein Sequence
MCGIFAYLNY HVPRTRREIL ETLIKGLQRL EYRGYDSAGV GLDGGNDKDW EANACKIQLI 60
KKKGKVKALD EEVHKQQDMD LDIEFDVHLG IAHTRWATHG EPNPVNSHPQ RSDKNNEFIV 120
IHNGIITNYK DLKKFLESKG YDFESETDTE TIAKLVKYMY DNWESQDVSF TTLVERVIQQ 180
LEGAFALVFK SVHFPGQAVG TRRGSPLLIG VRSEHKLSTD HIPILYRTAR TQIGSTWWGS 240
QAERGKDKKG SCGLSRVDST TCLFPVEEKA VEYYFASDAS AVIEHTNRVI FLEDDDVAAV 300
VDGRLSIHRI KRTAGDHPGR AVQTLQMELQ QIMKGNFSSF MQKEIFEQPE SVVNTMRGRV 360
NFDDYTVNLG GLKDHIKEIQ RCRRLILIAC GTSYHAGVAT RQVLEELTEL PVMVELASDF 420
LDRNTPVFRD DVCFFISQSG ETADTLMGLR YCKERGALTV GITNTVGSSI SRETDCGVHI 480
NAGPEIGVAS TKAYTSQFVS LVMFALMMCD DRISMQERRK EIMLGLKRLP DLIKEVLSMD 540
DEIQKLATEL YHQKSVLIMG RGYHYATCLE GALKIKEITY MHSEGILAGE LKHGPLALVD 600
KLMPVIMIIM RDHTYAKCQN ALQQVVARQG RPVVICDKED TETIKNTKRT IKVPHSVDCL 660
QGILSVIPLQ LLAFHLAVLR GYDVDFPRNL AKSVTVE 697 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0016597; F:amino acid binding; IEA:Compara.
 GO:0030246; F:carbohydrate binding; IEA:InterPro.
 GO:0004360; F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity; IEA:EC.
 GO:0016051; P:carbohydrate biosynthetic process; IEA:InterPro.
 GO:0032869; P:cellular response to insulin stimulus; IEA:Compara.
 GO:0006002; P:fructose 6-phosphate metabolic process; IEA:Compara.
 GO:0006042; P:glucosamine biosynthetic process; IEA:Compara.
 GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
 GO:0045719; P:negative regulation of glycogen biosynthetic process; IEA:Compara.
 GO:0051289; P:protein homotetramerization; IEA:Compara.
 GO:0009744; P:response to sucrose stimulus; IEA:Compara.
 GO:0006048; P:UDP-N-acetylglucosamine biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR017932; GATase_2_dom.
 IPR000583; GATase_dom.
 IPR005855; GlmS_trans.
 IPR001347; SIS. 
Pfam
 PF00310; GATase_2
 PF01380; SIS 
SMART
  
PROSITE
 PS51278; GATASE_TYPE_2
 PS51464; SIS 
PRINTS