CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001812
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Adenosine deaminase 
Protein Synonyms/Alias
 Adenosine aminohydrolase 
Gene Name
 ADA 
Gene Synonyms/Alias
 ADA1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
9AQTPAFDKPKVELHVubiquitination[1, 2]
54LNVIGMDKPLTLPDFacetylation[3]
54LNVIGMDKPLTLPDFubiquitination[2]
90AYEFVEMKAKEGVVYubiquitination[1, 2]
92EFVEMKAKEGVVYVEubiquitination[2]
111PHLLANSKVEPIPWNubiquitination[2]
147GERDFGVKARSILCCubiquitination[1, 2]
164HQPNWSPKVVELCKKacetylation[3]
164HQPNWSPKVVELCKKubiquitination[1, 2]
170PKVVELCKKYQQQTVubiquitination[1, 2]
171KVVELCKKYQQQTVVubiquitination[2]
225VGSAEVVKEAVDILKubiquitination[1, 2]
232KEAVDILKTERLGHGacetylation[3]
232KEAVDILKTERLGHGubiquitination[1, 2]
273SYLTGAWKPDTEHAVubiquitination[2]
284EHAVIRLKNDQANYSubiquitination[1, 2, 4]
301TDDPLIFKSTLDTDYubiquitination[2]
312DTDYQMTKRDMGFTEubiquitination[1, 2]
323GFTEEEFKRLNINAAubiquitination[1, 2]
331RLNINAAKSSFLPEDubiquitination[1, 2]
340SFLPEDEKRELLDLLubiquitination[1, 2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Catalyzes the hydrolytic deamination of adenosine and 2- deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte- epithelial cell adhesion. 
Sequence Annotation
 ACT_SITE 217 217 Proton donor (Probable).
 METAL 15 15 Zinc; catalytic.
 METAL 17 17 Zinc; catalytic.
 METAL 214 214 Zinc; catalytic.
 METAL 295 295 Zinc; catalytic.
 BINDING 17 17 Substrate.
 BINDING 19 19 Substrate.
 BINDING 184 184 Substrate; via amide nitrogen.
 BINDING 296 296 Substrate.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 54 54 N6-acetyllysine.
 MOD_RES 232 232 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Cell adhesion; Cell junction; Cell membrane; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Direct protein sequencing; Disease mutation; Hereditary hemolytic anemia; Hydrolase; Membrane; Metal-binding; Nucleotide metabolism; Polymorphism; Reference proteome; SCID; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 363 AA 
Protein Sequence
MAQTPAFDKP KVELHVHLDG SIKPETILYY GRRRGIALPA NTAEGLLNVI GMDKPLTLPD 60
FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV RYSPHLLANS KVEPIPWNQA 120
EGDLTPDEVV ALVGQGLQEG ERDFGVKARS ILCCMRHQPN WSPKVVELCK KYQQQTVVAI 180
DLAGDETIPG SSLLPGHVQA YQEAVKSGIH RTVHAGEVGS AEVVKEAVDI LKTERLGHGY 240
HTLEDQALYN RLRQENMHFE ICPWSSYLTG AWKPDTEHAV IRLKNDQANY SLNTDDPLIF 300
KSTLDTDYQM TKRDMGFTEE EFKRLNINAA KSSFLPEDEK RELLDLLYKA YGMPPSASAG 360
QNL 363 
Gene Ontology
 GO:0030054; C:cell junction; IEA:UniProtKB-SubCell.
 GO:0060205; C:cytoplasmic membrane-bounded vesicle lumen; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0032839; C:dendrite cytoplasm; IEA:Compara.
 GO:0009897; C:external side of plasma membrane; IDA:UniProtKB.
 GO:0005615; C:extracellular space; IEA:Compara.
 GO:0005764; C:lysosome; IDA:UniProtKB.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0004000; F:adenosine deaminase activity; IDA:UniProtKB.
 GO:0001883; F:purine nucleoside binding; IEA:Compara.
 GO:0008270; F:zinc ion binding; IMP:UniProtKB.
 GO:0006154; P:adenosine catabolic process; IDA:UniProtKB.
 GO:0007568; P:aging; IEA:Compara.
 GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
 GO:0046061; P:dATP catabolic process; IEA:Compara.
 GO:0006157; P:deoxyadenosine catabolic process; IEA:Compara.
 GO:0048566; P:embryonic digestive tract development; IEA:Compara.
 GO:0002314; P:germinal center B cell differentiation; IEA:Compara.
 GO:0001821; P:histamine secretion; IEA:Compara.
 GO:0043103; P:hypoxanthine salvage; IBA:RefGenome.
 GO:0046103; P:inosine biosynthetic process; ISS:UniProtKB.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0048286; P:lung alveolus development; IEA:Compara.
 GO:0060169; P:negative regulation of adenosine receptor signaling pathway; IDA:UniProtKB.
 GO:0042323; P:negative regulation of circadian sleep/wake cycle, non-REM sleep; IEA:Compara.
 GO:0050728; P:negative regulation of inflammatory response; IEA:Compara.
 GO:0002686; P:negative regulation of leukocyte migration; IEA:Compara.
 GO:0002906; P:negative regulation of mature B cell apoptotic process; IEA:Compara.
 GO:0070256; P:negative regulation of mucus secretion; IEA:Compara.
 GO:0060407; P:negative regulation of penile erection; IEA:Compara.
 GO:0070244; P:negative regulation of thymocyte apoptotic process; IEA:Compara.
 GO:0048541; P:Peyer's patch development; IEA:Compara.
 GO:0001890; P:placenta development; IEA:Compara.
 GO:0046638; P:positive regulation of alpha-beta T cell differentiation; IEA:Compara.
 GO:0030890; P:positive regulation of B cell proliferation; IEA:Compara.
 GO:0050850; P:positive regulation of calcium-mediated signaling; IEA:Compara.
 GO:0002636; P:positive regulation of germinal center formation; IEA:Compara.
 GO:0010460; P:positive regulation of heart rate; IEA:Compara.
 GO:0045987; P:positive regulation of smooth muscle contraction; IEA:Compara.
 GO:0033089; P:positive regulation of T cell differentiation in thymus; IEA:Compara.
 GO:0050862; P:positive regulation of T cell receptor signaling pathway; IEA:Compara.
 GO:0032261; P:purine nucleotide salvage; IMP:UniProtKB.
 GO:0009168; P:purine ribonucleoside monophosphate biosynthetic process; IEA:InterPro.
 GO:0033632; P:regulation of cell-cell adhesion mediated by integrin; IDA:UniProtKB.
 GO:0042542; P:response to hydrogen peroxide; IEA:Compara.
 GO:0001666; P:response to hypoxia; IDA:UniProtKB.
 GO:0043278; P:response to morphine; IEA:Compara.
 GO:0042110; P:T cell activation; IDA:UniProtKB.
 GO:0001829; P:trophectodermal cell differentiation; IEA:Compara.
 GO:0046111; P:xanthine biosynthetic process; IEA:Compara. 
Interpro
 IPR006650; A/AMP_deam_AS.
 IPR001365; A/AMP_deaminase_dom.
 IPR006330; Ado/ade_deaminase. 
Pfam
 PF00962; A_deaminase 
SMART
  
PROSITE
 PS00485; A_DEAMINASE 
PRINTS