| Tag | Content |
|---|
| CPLM ID | CPLM-005394 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | S-(hydroxymethyl)glutathione dehydrogenase |
Protein Synonyms/Alias | Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH |
Gene Name | frmA |
Gene Synonyms/Alias | adhC; b0356; JW0347 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 13 | AVAFAPGKPLEIVEI | acetylation | [1] | | 13 | AVAFAPGKPLEIVEI | pupylation | [2] | | 222 | IAIDTNPKKFDLARR | acetylation | [1] | | 243 | INPNDYDKPIKDVLL | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] [2] Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli. Cerda-Maira FA, McAllister F, Bode NJ, Burns KE, Gygi SP, Darwin KH. EMBO Rep. 2011 Jul 8;12(8):863-70. [ PMID: 21738222] |
Functional Description | Has high formaldehyde dehydrogenase activity in the presence of glutathione and catalyzes the oxidation of normal alcohols in a reaction that is not GSH-dependent. In addition, hemithiolacetals other than those formed from GSH, including omega-thiol fatty acids, also are substrates. |
Sequence Annotation | METAL 40 40 Zinc 1; catalytic (By similarity).
METAL 62 62 Zinc 1; catalytic (By similarity).
METAL 92 92 Zinc 2 (By similarity).
METAL 95 95 Zinc 2 (By similarity).
METAL 98 98 Zinc 2 (By similarity).
METAL 106 106 Zinc 2 (By similarity).
METAL 169 169 Zinc 1; catalytic (By similarity). |
Keyword | Complete proteome; Cytoplasm; Direct protein sequencing; Metal-binding; NAD; Oxidoreductase; Reference proteome; Zinc. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 369 AA |
Protein Sequence | MKSRAAVAFA PGKPLEIVEI DVAPPKKGEV LIKVTHTGVC HTDAFTLSGD DPEGVFPVVL 60
GHEGAGVVVE VGEGVTSVKP GDHVIPLYTA ECGECEFCRS GKTNLCVAVR ETQGKGLMPD 120
GTTRFSYNGQ PLYHYMGCST FSEYTVVAEV SLAKINPEAN HEHVCLLGCG VTTGIGAVHN 180
TAKVQPGDSV AVFGLGAIGL AVVQGARQAK AGRIIAIDTN PKKFDLARRF GATDCINPND 240
YDKPIKDVLL DINKWGIDHT FECIGNVNVM RAALESAHRG WGQSVIIGVA VAGQEISTRP 300
FQLVTGRVWK GSAFGGVKGR SQLPGMVEDA MKGDIDLEPF VTHTMSLDEI NDAFDLMHEG 360
KSIRTVIRY 369 |
Gene Ontology | GO:0005829; C:cytosol; IDA:UniProtKB. GO:0004024; F:alcohol dehydrogenase activity, zinc-dependent; IDA:EcoliWiki. GO:0051903; F:S-(hydroxymethyl)glutathione dehydrogenase activity; IDA:EcoliWiki. GO:0008270; F:zinc ion binding; IDA:EcoliWiki. GO:0006069; P:ethanol oxidation; IEA:InterPro. GO:0046294; P:formaldehyde catabolic process; IMP:EcoliWiki. |
Interpro | |
Pfam | |
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PRINTS | |