CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004289
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA ligase 
Protein Synonyms/Alias
 Polydeoxyribonucleotide synthase [NAD(+)] 
Gene Name
 ligA 
Gene Synonyms/Alias
 dnaL; lig; lop; pdeC; b2411; JW2403 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
48ELRELETKHPELITPacetylation[1]
183LPQAGFEKINEDARRacetylation[1]
457DVDGMGDKIIDQLVEacetylation[1]
465IIDQLVEKEYVHTPAacetylation[1]
481LFKLTAGKLTGLERMacetylation[1]
502NVVNALEKAKETTFAacetylation[1]
635VAGSVSKKTDLVIAGacetylation[1]
648AGEAAGSKLAKAQELacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. 
Sequence Annotation
 DOMAIN 593 671 BRCT.
 NP_BIND 32 36 NAD (Probable).
 NP_BIND 81 82 NAD.
 REGION 330 334 Interaction with target DNA.
 REGION 453 458 Interaction with target DNA.
 REGION 519 524 Interaction with target DNA.
 REGION 551 556 Interaction with target DNA.
 ACT_SITE 115 115 N6-AMP-lysine intermediate.
 METAL 408 408 Zinc.
 METAL 411 411 Zinc.
 METAL 426 426 Zinc.
 METAL 432 432 Zinc.
 BINDING 113 113 NAD.
 BINDING 136 136 NAD.
 BINDING 173 173 NAD (By similarity).
 BINDING 290 290 NAD.
 BINDING 314 314 NAD (Probable).  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; DNA replication; Ligase; Magnesium; Metal-binding; NAD; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 671 AA 
Protein Sequence
MESIEQQLTE LRTTLRHHEY LYHVMDAPEI PDAEYDRLMR ELRELETKHP ELITPDSPTQ 60
RVGAAPLAAF SQIRHEVPML SLDNVFDEES FLAFNKRVQD RLKNNEKVTW CCELKLDGLA 120
VSILYENGVL VSAATRGDGT TGEDITSNVR TIRAIPLKLH GENIPARLEV RGEVFLPQAG 180
FEKINEDARR TGGKVFANPR NAAAGSLRQL DPRITAKRPL TFFCYGVGVL EGGELPDTHL 240
GRLLQFKKWG LPVSDRVTLC ESAEEVLAFY HKVEEDRPTL GFDIDGVVIK VNSLAQQEQL 300
GFVARAPRWA VAFKFPAQEQ MTFVRDVEFQ VGRTGAITPV ARLEPVHVAG VLVSNATLHN 360
ADEIERLGLR IGDKVVIRRA GDVIPQVVNV VLSERPEDTR EVVFPTHCPV CGSDVERVEG 420
EAVARCTGGL ICGAQRKESL KHFVSRRAMD VDGMGDKIID QLVEKEYVHT PADLFKLTAG 480
KLTGLERMGP KSAQNVVNAL EKAKETTFAR FLYALGIREV GEATAAGLAA YFGTLEALEA 540
ASIEELQKVP DVGIVVASHV HNFFAEESNR NVISELLAEG VHWPAPIVIN AEEIDSPFAG 600
KTVVLTGSLS QMSRDDAKAR LVELGAKVAG SVSKKTDLVI AGEAAGSKLA KAQELGIEVI 660
DEAEMLRLLG S 671 
Gene Ontology
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003911; F:DNA ligase (NAD+) activity; IDA:EcoliWiki.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0006260; P:DNA replication; IEA:UniProtKB-KW. 
Interpro
 IPR001357; BRCT_dom.
 IPR018239; DNA_ligase_AS.
 IPR004150; DNA_ligase_OB.
 IPR001679; DNAligase.
 IPR013839; DNAligase_adenylation.
 IPR013840; DNAligase_N.
 IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
 IPR012340; NA-bd_OB-fold.
 IPR010994; RuvA_2-like.
 IPR004149; Znf_DNAligase_C4. 
Pfam
 PF00533; BRCT
 PF01653; DNA_ligase_aden
 PF03120; DNA_ligase_OB
 PF03119; DNA_ligase_ZBD 
SMART
 SM00292; BRCT
 SM00278; HhH1
 SM00532; LIGANc 
PROSITE
 PS50172; BRCT
 PS01055; DNA_LIGASE_N1
 PS01056; DNA_LIGASE_N2 
PRINTS