CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037258
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Nascent polypeptide-associated complex subunit alpha 
Protein Synonyms/Alias
  
Gene Name
 NACA 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
1941RSEKKARKAMSKLGLubiquitination[1]
1945KARKAMSKLGLRQVTubiquitination[1, 2, 3, 4]
1963RVTIRKSKNILFVITubiquitination[1, 2, 3, 4, 5, 6, 7, 8, 9, 10]
1971NILFVITKPDVYKSPacetylation[4, 11, 12]
1971NILFVITKPDVYKSPubiquitination[1, 2, 3, 4, 6, 9, 10]
1976ITKPDVYKSPASDTYubiquitination[4, 6, 10]
1990YIVFGEAKIEDLSQQubiquitination[6, 10]
2005AQLAAAEKFKVQGEAacetylation[4, 11, 12, 13]
2005AQLAAAEKFKVQGEAubiquitination[1, 2, 3, 4, 5, 6, 8, 9, 10, 14]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A data set of human endogenous protein ubiquitination sites.
 Shi Y, Chan DW, Jung SY, Malovannaya A, Wang Y, Qin J.
 Mol Cell Proteomics. 2011 May;10(5):M110.002089. [PMID: 20972266]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [10] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [11] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [12] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [13] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [14] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2078 AA 
Protein Sequence
MPGEATETVP ATEQELPQPQ AETAVLPMSS ALSVTAALGQ PGPTLPPPCS PAPQQCPLSA 60
ANQASPFPSP STIASTPLEV PFPQSSSGTA LPLGTAPEAP TFLPNLIGPP ISPAALALAS 120
PMIAPTLKGT PSSSAPLALV ALAPHSVQKS SAFPPNLLTS PPSVAVAESG SVITLSAPIA 180
PSEPKTNLNK VPSEVVPNPK GTPSPPCIVS TVPYHCVTPM ASIQSGVASL PQTTPTTTLA 240
IASPQVKDTT ISSVLISPQN PGSLSLKGPV SPPAALSLST QSLPVVTSSQ KTAGPNTPPD 300
FPISLGSHLA PLHQSSFGSV QLLGQTGPSA LSDPTVKTIS VDHSSTGASY PSQRSVIPPL 360
PSRNEVVPAT VAAFPVVAPS VDKGPSTISS ITCSPSGSLN VATSFSLSPT TSLILKSSPN 420
ATYHYPLVAQ MPVSSVGTTP LVVTNPCTIA AAPTTTFEVA TCVSPPMSSG PISNIEPTSP 480
AALVMAPVAP KEPSTQVATT LRIPVSPPLP DPEDLKNLPS SVLVKFPTQK DLQTVPASLE 540
GAPFSPAQAG LTTKKDPTVL PLVQAAPKNS PSFQSTSSSP EIPLSPEATL AKKSLGEPLP 600
IGKPASSMTS PLGVNSSASV IKTDSYAGPD SAGPLLKSSL ITPTVAAFPL ESADPAGVAP 660
TTAKGTSTYT TTASPFLEGT VSLAPKNHPV KEGTLTTLPL VPTASENCPV APSPQNTCAP 720
LATLVLAPEI PKSVPSPSLP PAGTPPGTKK VDGISHTSAL APVASSPKEC PTEDSGASAT 780
ASSKGTLTYL ADSPSPLGVS VSPQTKRPPT KKGSAGPDTP IGNLSSPVSP VEASFLPENS 840
LSFQGSKDSP ATTHSPTPPS PKGAPTPSAV TPLSPKGVTL PPKETPTPSV VNLPFPKEGP 900
ATPAPKQAPA LSMTSSSPKK ARATPAPKGI PASPSPKGAP TPPAATPPSP KGGPATPSPK 960
WAPTPPAATP PSPKGGPATP SPKGAPTPPA ATPPSPKGGP ATPSPKGAPT PPAVTPPSPK 1020
GSPAATPFPK GASTPPAATP PSPKGSPAAT PLPKGAPTTP AATLPSPKGG PATPSLKGAP 1080
TPPAATPPSP KGGPATPSPK GAPMPPAATP PSPKGGLATP PHKGAPTTPA ATPPSPKGGL 1140
ATPPPKGAPT TPAATPPSPK GGLATPPPKG APTTPAATPP SPKGGLATPS PKGAPTTPAA 1200
TPPSPKGGLA TPSPKGAPTT PAATPPSPKG GLATPSPKGA PTTPAATPPS PKGGPATPPP 1260
KGAPTPPAAT PPSLKGGLAT PPHKGAPNPA VVTPPSPKGG PATSPPKGAP TPPAATPPSP 1320
KGSPGTPPPK GAPTPPAVTP PSPKGTPTLP ATTPSSKGGP TTPSSKEGPT PPAATPSHKG 1380
GPAMTPPSPK RGPAIPSPKG DPTSPAVIPL SPKKAPATPV TREGAATPSK GDLTPPAVTP 1440
VSLKKAPATS APKGGPATPS SKGDPTLPAV TPPSPKEPPA PKQVATSSSP KKAPATPAPM 1500
GAPTLPAVIP SSPKEVPATP SSRRDPIAPT ATLLSKKTPA TLAPKEALIP PAMTVPSPKK 1560
TPAIPTPKEA PATPSSKEAS SPPAVTPSTY KGAPSPKELL IPPAVTSPSP KEAPTPPAVT 1620
PPSPEKGPAT PAPKGTPTSP PVTPSSLKDS PTSPASVTCK MGATVPQASK GLPAKKGPTA 1680
LKEVLVAPAP ESTPIITAPT RKGPQTKKSS ATSPPICPDP SAKNGSKGPL STVAPAPLLP 1740
VQKDSSKTAK GKDASHSPKG PLAPPESKAS TPLTAAAFEK VLPKPESASV SAAPSPPVSL 1800
PLAPSPVPTL PPKQQFLPSS PGLVLESPSK PLAPADEDEL LPLIPPEPIS GGVPFQSVLV 1860
NMPTPKSAGI PVPTPSAKQP VTKNNKGSGT ESDSDESVPE LEEQDSTQAT TQQAQLAAAA 1920
EIDEEPVSKA KQSRSEKKAR KAMSKLGLRQ VTGVTRVTIR KSKNILFVIT KPDVYKSPAS 1980
DTYIVFGEAK IEDLSQQAQL AAAEKFKVQG EAVSNIQENT QTPTVQEESE EEEVDETGVE 2040
VKDIELVMSQ ANVSRAKAVR ALKNNSNDIV NAIMELTM 2078 
Gene Ontology
  
Interpro
 IPR016641; EGD2/NACA.
 IPR002715; Nas_poly-pep-assoc_cplx_dom. 
Pfam
 PF01849; NAC 
SMART
  
PROSITE
 PS51151; NAC_AB 
PRINTS