UniProt Accession

 YGIC_ECOLI; P0ADT5; P24196; Q2M9G2 

Genbank Protein ID

 M77129; U28377; U00096; AP009048 

Genbank Nucleotide ID

 AAA71876.1; AAA69206.1; AAC76074.1; BAE77094.1 

Protein Name

 Putative acid--amine ligase YgiC 

Protein Synonyms/Alias

  

Gene Name

 ygiC 

Gene Synonyms/Alias

 b3038; JW3006 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
65	MCLKVVEKVIASDEL	acetylation	[1]
80	MTKFRIPKHTWSFVR	acetylation	[1]
247	LREMFSTKLEDAGVR	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 May be a ligase forming an amide bond. Shows ATPase activity. Despite its similarity to the C-terminal synthetase domain of Gss, is not a glutathionylspermidine (Gsp) synthetase. Cannot synthesize Gsp, glutathione (GSH), or GSH intermediates, from GSH and spermidine, cysteine and glutamate, gamma- glutamylcysteine and spermidine, and gamma-glutamylcysteine and glycine. Does not bind to Gsp. 

Sequence Annotation

 NP_BIND 100 102 ATP (By similarity).
 NP_BIND 371 373 ATP (By similarity).
 METAL 102 102 Magnesium 1 (By similarity).
 METAL 115 115 Magnesium 1 (By similarity).
 METAL 115 115 Magnesium 2 (By similarity).
 METAL 117 117 Magnesium 2 (By similarity).
 BINDING 267 267 ATP (By similarity).
 BINDING 302 302 ATP (By similarity).
 BINDING 309 309 ATP; via amide nitrogen (By similarity).
 BINDING 336 336 ATP (By similarity).  

Keyword

 ATP-binding; Complete proteome; Ligase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 386 AA 

Protein Sequence

Gene Ontology

 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. 

Interpro

 IPR005494; GSPS_pre-ATP-grasp-like_dom.
 IPR016185; PreATP-grasp_dom. 

Pfam

 PF03738; GSP_synth 

SMART

  

PROSITE

  

PRINTS