CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013317
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Anoctamin-6 
Protein Synonyms/Alias
 Small-conductance calcium-activated nonselective cation channel; SCAN channel; Transmembrane protein 16F 
Gene Name
 ANO6 
Gene Synonyms/Alias
 TMEM16F 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
77EFEEFNGKPDSLFFNubiquitination[1]
302HPRSIYKKQPLDLIRubiquitination[1]
665HRVSGSEKITPRWEQubiquitination[1]
814ADFKNKSKGNPYSDLubiquitination[1]
897REKYLTQKLLHENHLubiquitination[2]
909NHLKDMTKNMGVIAEubiquitination[1, 2]
929VDNNLRPKSE*****ubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Small-conductance calcium-activated nonselective cation (SCAN) channel which acts as a regulator of phospholipid scrambling in platelets and osteoblasts. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system whereas in osteoblasts is essential for the deposition of hydroxyapatite during bone mineralization. Can generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes. 
Sequence Annotation
 CARBOHYD 329 329 N-linked (GlcNAc...) (Potential).
 CARBOHYD 361 361 N-linked (GlcNAc...) (Potential).
 CARBOHYD 493 493 N-linked (GlcNAc...).
 CARBOHYD 777 777 N-linked (GlcNAc...) (Potential).
 CARBOHYD 790 790 N-linked (GlcNAc...) (Potential).
 CARBOHYD 802 802 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Cell membrane; Chloride; Chloride channel; Complete proteome; Glycoprotein; Ion channel; Ion transport; Membrane; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix; Transport; Voltage-gated channel. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 910 AA 
Protein Sequence
MKKMSRNVLL QMEEEEDDDD GDIGDVPASR RPFLTPHTHL PSSLVLENLG QTIVPDLGSL 60
ESQHDFRTPE FEEFNGKPDS LFFNDGQRRI DFVLVYEDES RKETNKKGTN EKQRRKRQAY 120
ESNLICHGLQ LEATRSVLDD KLVFVKVHAP WEVLCTYAEI MHIKLPLKPN DLKNRSSAFG 180
TLNWFTKVLS VDESIIKPEQ EFFTAPFEKN RMNDFYIVDR DAFFNPATRS RIVYFILSRV 240
KYQVINNVSK FGINRLVNSG IYKAAFPLHD CKFRRQSEDP SCPNERYLLY REWAHPRSIY 300
KKQPLDLIRK YYGEKIGIYF AWLGYYTQML LLAAVVGVAC FLYGYLNQDN CTWSKEVCHP 360
DIGGKIIMCP QCDRLCPFWK LNITCESSKK LCIFDSFGTL VFAVFMGVWV TLFLEFWKRR 420
QAELEYEWDT VELQQEEQAR PEYEARCTHV VINEITQEEE RIPFTAWGKC IRITLCASAV 480
FFWILLIIAS VIGIIVYRLS VFIVFSAKLP KNINGTDPIQ KYLTPQTATS ITASIISFII 540
IMILNTIYEK VAIMITNFEL PRTQTDYENS LTMKMFLFQF VNYYSSCFYI AFFKGKFVGY 600
PGDPVYWLGK YRNEECDPGG CLLELTTQLT IIMGGKAIWN NIQEVLLPWI MNLIGRFHRV 660
SGSEKITPRW EQDYHLQPMG KLGLFYEYLE MIIQFGFVTL FVASFPLAPL LALVNNILEI 720
RVDAWKLTTQ FRRLVPEKAQ DIGAWQPIMQ GIAILAVVTN AMIIAFTSDM IPRLVYYWSF 780
SVPPYGDHTS YTMEGYINNT LSIFKVADFK NKSKGNPYSD LGNHTTCRYR DFRYPPGHPQ 840
EYKHNIYYWH VIAAKLAFII VMEHVIYSVK FFISYAIPDV SKRTKSKIQR EKYLTQKLLH 900
ENHLKDMTKN MGVIAERMIE AVDNNLRPKS E 931 
Gene Ontology
 GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
 GO:0005622; C:intracellular; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0005227; F:calcium activated cation channel activity; ISS:UniProtKB.
 GO:0005254; F:chloride channel activity; IEA:UniProtKB-KW.
 GO:0005244; F:voltage-gated ion channel activity; ISS:UniProtKB.
 GO:0002543; P:activation of blood coagulation via clotting cascade; IMP:UniProtKB.
 GO:0007596; P:blood coagulation; ISS:UniProtKB.
 GO:0097045; P:phosphatidylserine exposure on blood platelet; IMP:UniProtKB. 
Interpro
 IPR007632; Anoctamin. 
Pfam
 PF04547; Anoctamin 
SMART
  
PROSITE
  
PRINTS