CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022478
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4 
Protein Synonyms/Alias
 Hormone-regulated proliferation-associated protein of 20 kDa 
Gene Name
 NDUFAF4 
Gene Synonyms/Alias
 C6orf66; HRPAP20; HSPC125; My013 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
51ISLYPEVKGEIARKDubiquitination[1, 2, 3, 4]
60EIARKDEKLLSFLKDubiquitination[1, 3]
66EKLLSFLKDVYVDSKubiquitination[1, 2, 3, 5]
73KDVYVDSKDPVSSLQubiquitination[2, 5, 6]
82PVSSLQVKAAETCQEubiquitination[6]
91AETCQEPKEFRLPKDacetylation[4]
91AETCQEPKEFRLPKDubiquitination[1, 3]
97PKEFRLPKDHHFDMIubiquitination[1, 3]
107HFDMINIKSIPKGKIubiquitination[1, 2, 3, 5, 6, 7]
113IKSIPKGKISIVEALubiquitination[5]
147QEYQLEQKDVNSLLKacetylation[8]
147QEYQLEQKDVNSLLKubiquitination[2, 6]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [8] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786
Functional Description
 Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). May be involved in cell proliferation and survival of hormone-dependent tumor cells. May be a regulator of breast tumor cell invasion. 
Sequence Annotation
  
Keyword
 Calmodulin-binding; Complete proteome; Disease mutation; Mitochondrion; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 175 AA 
Protein Sequence
MGALVIRGIR NFNLENRAER EISKMKPSVA PRHPSTNSLL REQISLYPEV KGEIARKDEK 60
LLSFLKDVYV DSKDPVSSLQ VKAAETCQEP KEFRLPKDHH FDMINIKSIP KGKISIVEAL 120
TLLNNHKLFP ETWTAEKIMQ EYQLEQKDVN SLLKYFVTFE VEIFPPEDKK AIRSK 175 
Gene Ontology
 GO:0031966; C:mitochondrial membrane; IDA:UniProtKB.
 GO:0032981; P:mitochondrial respiratory chain complex I assembly; IMP:UniProtKB. 
Interpro
 IPR009622; UPF0240. 
Pfam
 PF06784; UPF0240 
SMART
  
PROSITE
  
PRINTS