CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018840
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein Jumonji 
Protein Synonyms/Alias
 Jumonji/ARID domain-containing protein 2 
Gene Name
 JARID2 
Gene Synonyms/Alias
 JMJ 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
324AGVTSAKKMREVRPSubiquitination[1]
378VNHTISGKTESSNAKacetylation[2]
548KAEKGGGKAGWAAMDubiquitination[1]
583SVRAQVEKFGMCRVIubiquitination[1]
675MQQVTDLKKWNKLADubiquitination[1]
679TDLKKWNKLADMLRIubiquitination[1]
721EEHRRLEKEVLMEKEubiquitination[1]
727EKEVLMEKEILEKRKubiquitination[1]
757PLPRFEPKNGLIHGVubiquitination[1]
775NGFRSKLKEVGQAQLubiquitination[1]
783EVGQAQLKTGRRRLFubiquitination[1]
839IMSMCFSKEPAPAEIubiquitination[1]
857YWRLVEEKDCHVAVHubiquitination[1]
881GSGFPVGKSEPFSRHubiquitination[1]
1054MKRRHIAKPFSMEKLubiquitination[1]
1072IAQAEAKKENGPTLSubiquitination[1]
1124STVADGKKKPRKWLQubiquitination[1]
1179QKSCRGLKLMYRYDEubiquitination[1]
1199LVNQICGKVSGKNGSubiquitination[1]
1203ICGKVSGKNGSIENCubiquitination[1]
1213SIENCLSKPTPKRGPubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling. 
Sequence Annotation
 DOMAIN 557 598 JmjN.
 DOMAIN 621 713 ARID.
 DOMAIN 884 1048 JmjC.
 MOTIF 104 110 Nuclear localization signal (By
 MOTIF 874 878 GSGFP motif.
 MOD_RES 78 78 Phosphoserine.
 MOD_RES 378 378 N6-acetyllysine.
 MOD_RES 455 455 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Chromatin regulator; Complete proteome; Developmental protein; Differentiation; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1246 AA 
Protein Sequence
MSKERPKRNI IQKKYDDSDG IPWSEERVVR KVLYLSLKEF KNSQKRQHAE GIAGSLKTVN 60
GLLGNDQSKG LGPASEQSEN EKDDASQVSS TSNDVSSSDF EEGPSRKRPR LQAQRKFAQS 120
QPNSPSTTPV KIVEPLLPPP ATQISDLSKR KPKTEDFLTF LCLRGSPALP NSMVYFGSSQ 180
DEEEVEEEDD ETEDVKTATN NASSSCQSTP RKGKTHKHVH NGHVFNGSSR STREKEPVQK 240
HKSKEATPAK EKHSDHRADS RREQASANHP AAAPSTGSSA KGLAATHHHP PLHRSAQDLR 300
KQVSKVNGVT RMSSLGAGVT SAKKMREVRP SPSKTVKYTA TVTKGAVTYT KAKRELVKDT 360
KPNHHKPSSA VNHTISGKTE SSNAKTRKQV LSLGGASKST GPAVNGLKVS GRLNPKSCTK 420
EVGGRQLREG LQLREGLRNS KRRLEEAHQA EKPQSPPKKM KGAAGPAEGP GKKAPAERGL 480
LNGHVKKEVP ERSLERNRPK RATAGKSTPG RQAHGKADSA SCENRSTSQP ESVHKPQDSG 540
KAEKGGGKAG WAAMDEIPVL RPSAKEFHDP LIYIESVRAQ VEKFGMCRVI PPPDWRPECK 600
LNDEMRFVTQ IQHIHKLGRR WGPNVQRLAC IKKHLKSQGI TMDELPLIGG CELDLACFFR 660
LINEMGGMQQ VTDLKKWNKL ADMLRIPRTA QDRLAKLQEA YCQYLLSYDS LSPEEHRRLE 720
KEVLMEKEIL EKRKGPLEGH TENDHHKFHP LPRFEPKNGL IHGVAPRNGF RSKLKEVGQA 780
QLKTGRRRLF AQEKEVVKEE EEDKGVLNDF HKCIYKGRSV SLTTFYRTAR NIMSMCFSKE 840
PAPAEIEQEY WRLVEEKDCH VAVHCGKVDT NTHGSGFPVG KSEPFSRHGW NLTVLPNNTG 900
SILRHLGAVP GVTIPWLNIG MVFSTSCWSR DQNHLPYIDY LHTGADCIWY CIPAEEENKL 960
EDVVHTLLQA NGTPGLQMLE SNVMISPEVL CKEGIKVHRT VQQSGQFVVC FPGSFVSKVC 1020
CGYSVSETVH FATTQWTSMG FETAKEMKRR HIAKPFSMEK LLYQIAQAEA KKENGPTLST 1080
ISALLDELRD TELRQRRQLF EAGLHSSARY GSHDGSSTVA DGKKKPRKWL QLETSERRCQ 1140
ICQHLCYLSM VVQENENVVF CLECALRHVE KQKSCRGLKL MYRYDEEQII SLVNQICGKV 1200
SGKNGSIENC LSKPTPKRGP RKRATVDVPP SRLSASSSSK SASSSS 1246 
Gene Ontology
 GO:0035098; C:ESC/E(Z) complex; IEA:Compara.
 GO:0035097; C:histone methyltransferase complex; ISS:UniProtKB.
 GO:0003682; F:chromatin binding; ISS:UniProtKB.
 GO:0003677; F:DNA binding; IEA:Compara.
 GO:0007417; P:central nervous system development; TAS:ProtInc.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0008285; P:negative regulation of cell proliferation; IEA:Compara.
 GO:0031061; P:negative regulation of histone methylation; ISS:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; ISS:UniProtKB.
 GO:0051574; P:positive regulation of histone H3-K9 methylation; ISS:UniProtKB.
 GO:0048536; P:spleen development; IEA:Compara.
 GO:0048863; P:stem cell differentiation; ISS:UniProtKB.
 GO:0048538; P:thymus development; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001606; ARID/BRIGHT_DNA-bd.
 IPR003347; JmjC_dom.
 IPR003349; TF_JmjN.
 IPR004198; Znf_C5HC2. 
Pfam
 PF01388; ARID
 PF02373; JmjC
 PF02375; JmjN
 PF02928; zf-C5HC2 
SMART
 SM00501; BRIGHT
 SM00558; JmjC
 SM00545; JmjN 
PROSITE
 PS51011; ARID
 PS51184; JMJC
 PS51183; JMJN 
PRINTS