CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008283
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Carnitine O-palmitoyltransferase 1, liver isoform 
Protein Synonyms/Alias
 CPT1-L; Carnitine O-palmitoyltransferase I, liver isoform; CPT I; CPTI-L; Carnitine palmitoyltransferase 1A 
Gene Name
 CPT1A 
Gene Synonyms/Alias
 CPT1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
40LSGLHSWKKKFIRFKubiquitination[1, 2]
86PSLGIIAKINRTLETubiquitination[1, 2, 3, 4]
161VKIFSGRKPMLYSFQubiquitination[1, 2, 4]
180RLPVPAVKDTVNRYLubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
195QSVRPLMKEEDFKRMubiquitination[1, 2, 3, 4, 6, 7, 8]
292KLDREEIKPIRLLGSubiquitination[4]
405YFGRGKNKQSLDAVEubiquitination[3, 4, 7, 8]
441TSMDSYAKSLLHGRCubiquitination[4, 5, 7]
466FVVFKNGKMGLNAEHubiquitination[4]
634EQRLKLFKLASEKHQubiquitination[4]
675AVESPFLKEVLSEPWubiquitination[3, 4, 5, 6, 7, 8]
755HRFGRHLKEAMTDIIubiquitination[4]
772FGLSSNSKK******ubiquitination[3, 4, 7]
773GLSSNSKK*******ubiquitination[4, 7]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Plays an important role in triglyceride metabolism. 
Sequence Annotation
 REGION 555 567 Coenzyme A binding (By similarity).
 ACT_SITE 473 473 Proton acceptor (By similarity).
 BINDING 589 589 Carnitine (By similarity).
 BINDING 602 602 Carnitine (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 282 282 Nitrated tyrosine (By similarity).
 MOD_RES 588 588 Phosphothreonine (By similarity).
 MOD_RES 589 589 Nitrated tyrosine (By similarity).
 MOD_RES 604 604 Phosphothreonine (By similarity).
 MOD_RES 741 741 Phosphoserine (By similarity).
 MOD_RES 747 747 Phosphoserine (By similarity).  
Keyword
 3D-structure; Acetylation; Acyltransferase; Alternative splicing; Complete proteome; Disease mutation; Fatty acid metabolism; Lipid metabolism; Membrane; Mitochondrion; Mitochondrion outer membrane; Nitration; Phosphoprotein; Polymorphism; Reference proteome; Transferase; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 773 AA 
Protein Sequence
MAEAHQAVAF QFTVTPDGID LRLSHEALRQ IYLSGLHSWK KKFIRFKNGI ITGVYPASPS 60
SWLIVVVGVM TTMYAKIDPS LGIIAKINRT LETANCMSSQ TKNVVSGVLF GTGLWVALIV 120
TMRYSLKVLL SYHGWMFTEH GKMSRATKIW MGMVKIFSGR KPMLYSFQTS LPRLPVPAVK 180
DTVNRYLQSV RPLMKEEDFK RMTALAQDFA VGLGPRLQWY LKLKSWWATN YVSDWWEEYI 240
YLRGRGPLMV NSNYYAMDLL YILPTHIQAA RAGNAIHAIL LYRRKLDREE IKPIRLLGST 300
IPLCSAQWER MFNTSRIPGE ETDTIQHMRD SKHIVVYHRG RYFKVWLYHD GRLLKPREME 360
QQMQRILDNT SEPQPGEARL AALTAGDRVP WARCRQAYFG RGKNKQSLDA VEKAAFFVTL 420
DETEEGYRSE DPDTSMDSYA KSLLHGRCYD RWFDKSFTFV VFKNGKMGLN AEHSWADAPI 480
VAHLWEYVMS IDSLQLGYAE DGHCKGDINP NIPYPTRLQW DIPGECQEVI ETSLNTANLL 540
ANDVDFHSFP FVAFGKGIIK KCRTSPDAFV QLALQLAHYK DMGKFCLTYE ASMTRLFREG 600
RTETVRSCTT ESCDFVRAMV DPAQTVEQRL KLFKLASEKH QHMYRLAMTG SGIDRHLFCL 660
YVVSKYLAVE SPFLKEVLSE PWRLSTSQTP QQQVELFDLE NNPEYVSSGG GFGPVADDGY 720
GVSYILVGEN LINFHISSKF SCPETDSHRF GRHLKEAMTD IITLFGLSSN SKK 773 
Gene Ontology
 GO:0031307; C:integral to mitochondrial outer membrane; ISS:UniProtKB.
 GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
 GO:0004095; F:carnitine O-palmitoyltransferase activity; IDA:UniProtKB.
 GO:0009437; P:carnitine metabolic process; IDA:UniProtKB.
 GO:0006853; P:carnitine shuttle; TAS:Reactome.
 GO:0042755; P:eating behavior; IEA:Compara.
 GO:0006635; P:fatty acid beta-oxidation; TAS:ProtInc.
 GO:0006006; P:glucose metabolic process; IEA:Compara.
 GO:0001676; P:long-chain fatty acid metabolic process; IDA:UniProtKB.
 GO:0032000; P:positive regulation of fatty acid beta-oxidation; IEA:Compara.
 GO:0051260; P:protein homooligomerization; IEA:Compara.
 GO:0050796; P:regulation of insulin secretion; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0014070; P:response to organic cyclic compound; IEA:Compara.
 GO:0006641; P:triglyceride metabolic process; IEA:Compara. 
Interpro
 IPR000542; Carn_acyl_trans. 
Pfam
 PF00755; Carn_acyltransf 
SMART
  
PROSITE
 PS00439; ACYLTRANSF_C_1
 PS00440; ACYLTRANSF_C_2 
PRINTS