CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-036953
UniProt Accession
Genbank Protein ID
 U52112 
Genbank Nucleotide ID
  
Protein Name
 Rho GTPase-activating protein 4 
Protein Synonyms/Alias
  
Gene Name
 ARHGAP4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
22AEYETQVKEMRWQLSubiquitination[1, 2]
215QAKFMEHKLKCTKARubiquitination[1]
217KFMEHKLKCTKARNEubiquitination[1]
360IETEEVNKTLKATLQubiquitination[1, 2]
422TETFYLTKLQEYLSGubiquitination[1, 3, 4]
435SGRSILAKLQAKHEKubiquitination[1]
442KLQAKHEKLQEALQRubiquitination[1, 2]
452EALQRGDKEEQEVSWubiquitination[1]
490LFGGDMEKFIQSSGQubiquitination[1]
777MRGLIPHKYITLPAGubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 925 AA 
Protein Sequence
MAAHGKLRRE RGLQAEYETQ VKEMRWQLSE QLRCLELQGE LRRELLQELA EFMRRRAEVE 60
LEYSRGLEKL AERFSSRGGR LGSSREHQSF RKEPSLLSPL HCWAVLLQHT RQQSRESAAL 120
SEVLAGPLAQ RLSHIAEDVG RLVKKAKKTY QAYHMESVNA EAKLREAERQ EEKRAGRSVP 180
TTTAGATEAG PLRKSSLKKG GRLVEKRQAK FMEHKLKCTK ARNEYLLSLA SVNAAVSNYY 240
LHDVLDLMDC CDTGFHLALG QVLRSYTAAE SRTQASQVQG LGSLEEAVEA LDPPGDKAKV 300
LEVHATVFCP PLRFDYHPHD GDEVAEICVE MELRDEILPR AQNIQSRLDR QTIETEEVNK 360
TLKATLQALL EVVASDDGDV LDSFQTSPST ESLKSTSSDP GSRQAGRRRG QQQETETFYL 420
TKLQEYLSGR SILAKLQAKH EKLQEALQRG DKEEQEVSWT QYTQRKFQKS RQPRPSSQYN 480
QRLFGGDMEK FIQSSGQPVP LVVESCIRFI NLNGLQHEGI FRVSGAQLRV SEIRDAFERG 540
EDPLVEGCTA HDLDSVAGVL KLYFRSLEPP LFPPDLFGEL LASSELEATA ERVEHVSRLL 600
WRLPAPVLVV LRYLFTFLNH LAQYSDENMM DPYNLAVCFG PTLLPVPAGQ DPVALQGRVN 660
QLVQTLIVQP DRVFPPLTSL PGPVYEKCMA PPSASCLGDA QLESLGADNE PELEAEMPAQ 720
EDDLEGVVEA VACFAYTGRT AQELSFRRGD VLRLHERASS DWWRGEHNGM RGLIPHKYIT 780
LPAGTEKQVV GAGLQTAGES GSSPEGLLAS ELVHRPEPCT SPEAMGPSGH RRRCLVPASP 840
EQHVEVDKAV AQNMDSVFKE LLGKTSVRQG LGPASTTSPS PGPRSPKAPP SSRLGRNKGF 900
SRGPGAPASP SASHPQGLDT TPKPH 925 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005925; C:focal adhesion; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0007165; P:signal transduction; IEA:InterPro. 
Interpro
 IPR001060; FCH_dom.
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom.
 IPR001452; SH3_domain. 
Pfam
 PF00611; FCH
 PF00620; RhoGAP
 PF00018; SH3_1 
SMART
 SM00055; FCH
 SM00324; RhoGAP
 SM00326; SH3 
PROSITE
 PS50133; FCH
 PS50238; RHOGAP
 PS50002; SH3 
PRINTS