UniProt Accession

 PTN1_HUMAN; P18031; Q5TGD8; Q9BQV9; Q9NQQ4 

Genbank Protein ID

 M31724; M33689; M33684; M33688; M33687; M33686; M33685; BT006752; AL133230; AL034429; AL034429; AL133230; BC015660; BC018164 

Genbank Nucleotide ID

 AAA60223.1; AAA60157.1; AAA60158.1; AAA60158.1; AAA60158.1; AAA60158.1; AAA60158.1; AAP35398.1; CAC00618.2; CAC00618.2; CAI23215.1; CAI23215.1; AAH15660.1; AAH18164.1 

Protein Name

 Tyrosine-protein phosphatase non-receptor type 1 

Protein Synonyms/Alias

 Protein-tyrosine phosphatase 1B; PTP-1B 

Gene Name

 PTPN1 

Gene Synonyms/Alias

 PTP1B 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
5	***MEMEKEFEQIDK	ubiquitination	[1]
58	PFDHSRIKLHQEDND	ubiquitination	[1]
239	LLLMDKRKDPSSVDI	ubiquitination	[1]
248	PSSVDIKKVLLEMRK	ubiquitination	[1]
335	FPNHQWVKEETQEDK	sumoylation	[2]
347	EDKDCPIKEEKGSPL	sumoylation	[2]
350	DCPIKEEKGSPLNAA	ubiquitination	[3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Regulation of protein tyrosine phosphatase 1B by sumoylation.
 Dadke S, Cotteret S, Yip SC, Jaffer ZM, Haj F, Ivanov A, Rauscher F 3rd, Shuai K, Ng T, Neel BG, Chernoff J.
 Nat Cell Biol. 2007 Jan;9(1):80-5. [PMID: 17159996]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. 

Sequence Annotation

 DOMAIN 3 277 Tyrosine-protein phosphatase.
 REGION 215 221 Substrate binding (By similarity).
 ACT_SITE 215 215 Phosphocysteine intermediate.
 BINDING 181 181 Substrate.
 BINDING 262 262 Substrate (By similarity).
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 20 20 Phosphotyrosine.
 MOD_RES 50 50 Phosphoserine; by PKB/AKT1, CLK1 and
 MOD_RES 66 66 Phosphotyrosine; by EGFR.
 MOD_RES 215 215 Cysteine persulfide.
 MOD_RES 215 215 S-nitrosocysteine; in reversibly
 MOD_RES 242 242 Phosphoserine; by CLK1 and CLK2.
 MOD_RES 243 243 Phosphoserine; by CLK1 and CLK2.
 MOD_RES 352 352 Phosphoserine.
 MOD_RES 378 378 Phosphoserine; by PKC.
 MOD_RES 386 386 Phosphoserine; by CDK1.
 MOD_RES 393 393 Phosphoserine (By similarity).
 CROSSLNK 215 216 N,N-(cysteine-1,S-diyl)serine (Cys-Ser);  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Hydrolase; Membrane; Oxidation; Phosphoprotein; Polymorphism; Protein phosphatase; Reference proteome; S-nitrosylation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 435 AA 

Protein Sequence

Gene Ontology

 GO:0031410; C:cytoplasmic vesicle; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005769; C:early endosome; IDA:UniProtKB.
 GO:0005783; C:endoplasmic reticulum; IDA:HPA.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0004725; F:protein tyrosine phosphatase activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IDA:UniProtKB.
 GO:0031532; P:actin cytoskeleton reorganization; IMP:UniProtKB.
 GO:0030968; P:endoplasmic reticulum unfolded protein response; IDA:UniProtKB.
 GO:0008286; P:insulin receptor signaling pathway; IEA:Compara.
 GO:0060333; P:interferon-gamma-mediated signaling pathway; TAS:Reactome.
 GO:0060397; P:JAK-STAT cascade involved in growth hormone signaling pathway; TAS:Reactome.
 GO:0046627; P:negative regulation of insulin receptor signaling pathway; NAS:BHF-UCL.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0030100; P:regulation of endocytosis; IDA:UniProtKB.
 GO:0060334; P:regulation of interferon-gamma-mediated signaling pathway; TAS:Reactome.
 GO:0060338; P:regulation of type I interferon-mediated signaling pathway; TAS:Reactome.
 GO:0060337; P:type I interferon-mediated signaling pathway; TAS:Reactome. 

Interpro

 IPR012265; Ptpn1/Ptpn2.
 IPR000387; Tyr/Dual-sp_Pase.
 IPR016130; Tyr_Pase_AS.
 IPR000242; Tyr_Pase_rcpt/non-rcpt. 

Pfam

 PF00102; Y_phosphatase 

SMART

 SM00194; PTPc 

PROSITE

 PS00383; TYR_PHOSPHATASE_1
 PS50056; TYR_PHOSPHATASE_2
 PS50055; TYR_PHOSPHATASE_PTP 

PRINTS

 PR00700; PRTYPHPHTASE.