UniProt Accession

 DHYS_HUMAN; P49366; A8K688; M0R1I5; Q13184; Q13276; Q9UDG0 

Genbank Protein ID

 L39068; U40579; U32178; U26266; AJ001701; AJ001702; AJ001703; AJ001704; U79262; AL520040; AK291553; AC010422; CH471106; BC000333; BC014016 

Genbank Nucleotide ID

 AAA86282.1; AAA96151.1; AAB02179.1; AAB02175.1; CAA04940.1; CAA04940.1; CAA04940.1; CAA04940.1; AAB50208.1; BAF84242.1; EAW84288.1; AAH00333.1; AAH14016.1 

Protein Name

 Deoxyhypusine synthase 

Protein Synonyms/Alias

 DHS 

Gene Name

 DHPS 

Gene Synonyms/Alias

 DS 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
19	GALAAVLKHSSTLPP	ubiquitination	[1, 2, 3, 4]
72	QVNAMIEKKLEPLSQ	ubiquitination	[3, 5]
73	VNAMIEKKLEPLSQD	ubiquitination	[3, 5]
156	GEFSLRGKELRENGI	ubiquitination	[3]
205	GVKWTPSKMIARLGK	ubiquitination	[2, 3, 5]
212	KMIARLGKEINNPES	ubiquitination	[2, 3, 5]
329	DEAVSWGKIRVDAQP	ubiquitination	[3]
366	MDAFMHEKNED****	ubiquitination	[2, 3, 5]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. 

Sequence Annotation

 NP_BIND 105 109 NAD.
 NP_BIND 131 133 NAD.
 NP_BIND 308 309 NAD.
 NP_BIND 342 343 NAD.
 REGION 136 137 Spermidine binding (Probable).
 REGION 314 316 Spermidine binding (Probable).
 REGION 323 329 Spermidine binding (Probable).
 ACT_SITE 329 329 Nucleophile.
 BINDING 137 137 NAD.
 BINDING 238 238 NAD.
 BINDING 243 243 Spermidine (Probable).
 BINDING 283 283 NAD; via amide nitrogen.
 BINDING 288 288 Spermidine (Probable).
 MOD_RES 78 78 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Direct protein sequencing; Hypusine biosynthesis; NAD; Phosphoprotein; Polymorphism; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 369 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0034038; F:deoxyhypusine synthase activity; TAS:Reactome.
 GO:0050983; P:deoxyhypusine biosynthetic process from spermidine; TAS:ProtInc.
 GO:0008612; P:peptidyl-lysine modification to hypusine; TAS:Reactome.
 GO:0008284; P:positive regulation of cell proliferation; TAS:ProtInc.
 GO:0043687; P:post-translational protein modification; TAS:Reactome.
 GO:0051289; P:protein homotetramerization; IEA:Compara.
 GO:0006412; P:translation; TAS:ProtInc. 

Interpro

 IPR002773; Deoxyhypusine_synthase. 

Pfam

 PF01916; DS 

SMART

  

PROSITE

  

PRINTS