CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005261
UniProt Accession
Genbank Protein ID
 M59742 
Genbank Nucleotide ID
Protein Name
 Sodium- and chloride-dependent GABA transporter 1 
Protein Synonyms/Alias
 GAT-1; Solute carrier family 6 member 1 
Gene Name
 Slc6a1 
Gene Synonyms/Alias
 Gabt1; Gat-1; Gat1 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
7*MATDNSKVADGQISubiquitination[1]
26EAPVASDKPKTLVVKacetylation[2]
28PVASDKPKTLVVKVQubiquitination[1]
33KPKTLVVKVQKKAGDubiquitination[1]
37LVVKVQKKAGDLPDRubiquitination[1]
595QAGSSASKEAYI***ubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals. 
Sequence Annotation
 MOTIF 597 599 PDZ-binding.
 CARBOHYD 176 176 N-linked (GlcNAc...) (Potential).
 CARBOHYD 181 181 N-linked (GlcNAc...) (Potential).
 CARBOHYD 184 184 N-linked (GlcNAc...) (Potential).  
Keyword
 Cell membrane; Complete proteome; Direct protein sequencing; Glycoprotein; Membrane; Neurotransmitter transport; Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 599 AA 
Protein Sequence
MATDNSKVAD GQISTEVSEA PVASDKPKTL VVKVQKKAGD LPDRDTWKGR FDFLMSCVGY 60
AIGLGNVWRF PYLCGKNGGG AFLIPYFLTL IFAGVPLFLL ECSLGQYTSI GGLGVWKLAP 120
MFKGVGLAAA VLSFWLNIYY IVIISWAIYY LYNSFTTTLP WKQCDNPWNT DRCFSNYSLV 180
NTTNMTSAVV EFWERNMHQM TDGLDKPGQI RWPLAITLAI AWVLVYFCIW KGVGWTGKVV 240
YFSATYPYIM LIILFFRGVT LPGAKEGILF YITPNFRKLS DSEVWLDAAT QIFFSYGLGL 300
GSLIALGSYN SFHNNVYRDS IIVCCINSCT SMFAGFVIFS IVGFMAHVTK RSIADVAASG 360
PGLAFLAYPE AVTQLPISPL WAILFFSMLL MLGIDSQFCT VEGFITALVD EYPRLLRNRR 420
ELFIAAVCIV SYLIGLSNIT QGGIYVFKLF DYYSASGMSL LFLVFFECVS ISWFYGVNRF 480
YDNIQEMVGS RPCIWWKLCW SFFTPIIVAG VFLFSAVQMT PLTMGSYVFP KWGQGVGWLM 540
ALSSMVLIPG YMAYMFLTLK GSLKQRLQVM IQPSEDIVRP ENGPEQPQAG SSASKEAYI 599 
Gene Ontology
 GO:0030424; C:axon; IEA:Compara.
 GO:0009986; C:cell surface; IDA:RGD.
 GO:0005887; C:integral to plasma membrane; IEA:InterPro.
 GO:0043005; C:neuron projection; IDA:RGD.
 GO:0005886; C:plasma membrane; IDA:RGD.
 GO:0005332; F:gamma-aminobutyric acid:sodium symporter activity; IDA:RGD.
 GO:0005328; F:neurotransmitter:sodium symporter activity; IEA:InterPro.
 GO:0051939; P:gamma-aminobutyric acid import; IDA:RGD.
 GO:0007612; P:learning; IMP:RGD.
 GO:0032229; P:negative regulation of synaptic transmission, GABAergic; IMP:RGD.
 GO:0014054; P:positive regulation of gamma-aminobutyric acid secretion; IMP:RGD.
 GO:0051260; P:protein homooligomerization; IDA:RGD.
 GO:0051592; P:response to calcium ion; IEP:RGD.
 GO:0042220; P:response to cocaine; IEP:RGD.
 GO:0032355; P:response to estradiol stimulus; IEP:RGD.
 GO:0010288; P:response to lead ion; IEP:RGD.
 GO:0014074; P:response to purine-containing compound; IMP:RGD.
 GO:0009744; P:response to sucrose stimulus; IEP:RGD.
 GO:0009636; P:response to toxic substance; IMP:RGD. 
Interpro
 IPR000175; Na/ntran_symport.
 IPR002980; Na/ntran_symport_GABA_GAT1. 
Pfam
 PF00209; SNF 
SMART
  
PROSITE
 PS00610; NA_NEUROTRAN_SYMP_1
 PS00754; NA_NEUROTRAN_SYMP_2
 PS50267; NA_NEUROTRAN_SYMP_3 
PRINTS
 PR01195; GAT1TRNSPORT.
 PR00176; NANEUSMPORT.