UniProt Accession

 UBA3_HUMAN; Q8TBC4; A6NLB5; A8K027; O76088; Q9NTU3 

Genbank Protein ID

 AL117566; AK002159; AK289392; AC092060; AC109587; CH471055; BC022853; AF046024; AB012190 

Genbank Nucleotide ID

 CAB55996.2; BAG51021.1; BAF82081.1; EAW65470.1; AAH22853.1; AAC27648.1; BAA33144.1 

Protein Name

 NEDD8-activating enzyme E1 catalytic subunit 

Protein Synonyms/Alias

 NEDD8-activating enzyme E1C; Ubiquitin-activating enzyme E1C; Ubiquitin-like modifier-activating enzyme 3; Ubiquitin-activating enzyme 3 

Gene Name

 UBA3 

Gene Synonyms/Alias

 UBE1C 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
246	VRMLQWPKEQPFGEG	ubiquitination	[1]
270	HIQWIFQKSLERASQ	ubiquitination	[1]
293	RLTQGVVKRIIPAVA	ubiquitination	[1]
384	NSASLQMKSPAITAT	ubiquitination	[1]
395	ITATLEGKNRTLYLQ	ubiquitination	[1]

Reference

 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. 

Sequence Annotation

 NP_BIND 100 124 ATP.
 NP_BIND 148 171 ATP.
 REGION 53 70 Interaction with UBE2M N-terminus.
 REGION 157 161 Interaction with UBE2M N-terminus.
 REGION 192 217 Interaction with UBE2M N-terminus.
 REGION 227 229 Interaction with NEDD8.
 REGION 242 248 Interaction with NAE1.
 REGION 292 295 Interaction with NAE1.
 REGION 331 338 Interaction with UBE2M N-terminus.
 REGION 352 357 Interaction with NEDD8.
 REGION 368 463 Interaction with UBE2M core domain.
 ACT_SITE 237 237 Glycyl thioester intermediate.  

Keyword

 3D-structure; Alternative splicing; ATP-binding; Cell cycle; Complete proteome; Ligase; Nucleotide-binding; Polymorphism; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 463 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IDA:LIFEdb.
 GO:0016881; F:acid-amino acid ligase activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0019781; F:NEDD8 activating enzyme activity; IEA:Compara.
 GO:0006464; P:cellular protein modification process; TAS:ProtInc.
 GO:0007113; P:endomitotic cell cycle; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0045116; P:protein neddylation; IEA:UniProtKB-UniPathway.
 GO:0006508; P:proteolysis; TAS:ProtInc.
 GO:0051726; P:regulation of cell cycle; IEA:Compara. 

Interpro

 IPR014929; E2_binding.
 IPR009036; Molybdenum_cofac_synth_MoeB.
 IPR016040; NAD(P)-bd_dom.
 IPR000594; ThiF_NAD_FAD-bd.
 IPR023318; Ub_act_enz_dom_a.
 IPR000127; UBact_repeat.
 IPR019572; Ubiquitin-activating_enzyme.
 IPR018074; UBQ-activ_enz_E1_AS. 

Pfam

 PF08825; E2_bind
 PF00899; ThiF
 PF10585; UBA_e1_thiolCys
 PF02134; UBACT 

SMART

  

PROSITE

 PS00536; UBIQUITIN_ACTIVAT_1
 PS00865; UBIQUITIN_ACTIVAT_2 

PRINTS