CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001313
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nebulette 
Protein Synonyms/Alias
 Actin-binding Z-disk protein 
Gene Name
 NEBL 
Gene Synonyms/Alias
 LNEBL 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
525ASQKQYKKDLENEIKacetylation[1]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Binds to actin and plays an important role in the assembly of the Z-disk. Isoform 2 might play a role in the assembly of focal adhesion. 
Sequence Annotation
 REPEAT 29 63 Nebulin 1.
 REPEAT 64 99 Nebulin 2.
 REPEAT 100 136 Nebulin 3.
 REPEAT 137 172 Nebulin 4.
 REPEAT 173 205 Nebulin 5.
 REPEAT 206 241 Nebulin 6.
 REPEAT 242 278 Nebulin 7.
 REPEAT 279 313 Nebulin 8.
 REPEAT 314 348 Nebulin 9.
 REPEAT 349 385 Nebulin 10.
 REPEAT 386 422 Nebulin 11.
 REPEAT 423 459 Nebulin 12.
 REPEAT 460 496 Nebulin 13.
 REPEAT 497 533 Nebulin 14.
 REPEAT 534 569 Nebulin 15.
 REPEAT 570 599 Nebulin 16.
 REPEAT 600 635 Nebulin 17.
 REPEAT 636 666 Nebulin 18.
 REPEAT 667 693 Nebulin 19.
 REPEAT 694 728 Nebulin 20.
 REPEAT 729 759 Nebulin 21.
 REPEAT 760 794 Nebulin 22.
 REPEAT 795 830 Nebulin 23.
 DOMAIN 954 1014 SH3.
 REGION 836 953 Linker.  
Keyword
 3D-structure; Actin-binding; Alternative splicing; Complete proteome; Cytoplasm; Polymorphism; Reference proteome; Repeat; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1014 AA 
Protein Sequence
MRVPVFEDIK DETEEEKIGE EENEEDQVFY KPVIEDLSME LARKCTELIS DIRYKEEFKK 60
SKDKCTFVTD SPMLNHVKNI GAFISEAKYK GTIKADLSNS LYKRMPATID SVFAGEVTQL 120
QSEVAYKQKH DAAKGFSDYA HMKEPPEVKH AMEVNKHQSN ISYRKDVQDT HTYSAELDRP 180
DIKMATQISK IISNAEYKKG QGIMNKEPAV IGRPDFEHAV EASKLSSQIK YKEKFDNEMK 240
DKKHHYNPLE SASFRQNQLA ATLASNVKYK KDIQNMHDPV SDLPNLLFLD HVLKASKMLS 300
GREYKKLFEE NKGMYHFDAD AVEHLHHKGN AVLQSQVKYK EEYEKNKGKP MLEFVETPSY 360
QASKEAQKMQ SEKVYKEDFE KEIKGRSSLD LDKTPEFLHV KYITNLLREK EYKKDLENEI 420
KGKGMELNSE VLDIQRAKRA SEMASEKEYK KDLESIIKGK GMQAGTDTLE MQHAKKAAEI 480
ASEKDYKRDL ETEIKGKGMQ VSTDTLDVQR AKKASEMASQ KQYKKDLENE IKGKGMQVSM 540
DIPDILRAKR TSEIYSQRKY KDEAEKMLSN YSTIADTPEI QRIKTTQQNI SAVFYKKEVG 600
AGTAVKDSPE IERVKKNQQN ISSVKYKEEI KHATAISDPP ELKRVKENQK NISNLQYKEQ 660
NYKATPVSMT PEIERVRRNQ EQLSAVKYKG ELQRGTAISD PPELKRAKEN QKNISNVYYR 720
GQLGRATTLS VTPEMERVKK NQENISSVKY TQDHKQMKGR PSLILDTPAM RHVKEAQNHI 780
SMVKYHEDFE KTKGRGFTPV VDDPVTERVR KNTQVVSDAA YKGVHPHIVE MDRRPGIIVD 840
LKVWRTDPGS IFDLDPLEDN IQSRSLHMLS EKASHYRRHW SRSHSSSTFG TGLGDDRSEI 900
SEIYPSFSCC SEVTRPSDEG APVLPGAYQQ SHSQGYGYMH QTSVSSMRSM QHSPNLRTYR 960
AMYDYSAQDE DEVSFRDGDY IVNVQPIDDG WMYGTVQRTG RTGMLPANYI EFVN 1014 
Gene Ontology
 GO:0001725; C:stress fiber; IDA:UniProtKB.
 GO:0030018; C:Z disc; IDA:UniProtKB.
 GO:0051015; F:actin filament binding; IDA:UniProtKB.
 GO:0008307; F:structural constituent of muscle; NAS:UniProtKB.
 GO:0071691; P:cardiac muscle thin filament assembly; IMP:UniProtKB. 
Interpro
 IPR000900; Nebulin_35r-motif.
 IPR001452; SH3_domain. 
Pfam
 PF00880; Nebulin
 PF00018; SH3_1 
SMART
 SM00227; NEBU
 SM00326; SH3 
PROSITE
 PS51216; NEBULIN
 PS50002; SH3 
PRINTS
 PR00452; SH3DOMAIN.