CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007466
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 2D 
Protein Synonyms/Alias
 eIF2d; Hepatocellular carcinoma-associated antigen 56; Ligatin 
Gene Name
 EIF2D 
Gene Synonyms/Alias
 HCA56; LGTN 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
4****MFAKAFRVKSNubiquitination[1]
333QEQIIQVKELSKGVEacetylation[2, 3, 4]
337IQVKELSKGVESIVAubiquitination[1]
446LCDCILEKNEQHTVMubiquitination[1]
454NEQHTVMKLPWDSLLubiquitination[1]
485GQEPIVKKGRICPIDubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Translation initiation factor that is able to deliver tRNA to the P-site of the eukaryotic ribosome in a GTP-independent manner. The binding of Met-tRNA(I) occurs after the AUG codon finds its position in the P-site of 40S ribosomes, the situation that takes place during initiation complex formation on some specific RNAs. Its activity in tRNA binding with 40S subunits does not require the presence of the aminoacyl moiety. Possesses the unique ability to deliver non-Met (elongator) tRNAs into the P- site of the 40S subunit. In addition to its role in initiation, can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits. 
Sequence Annotation
 DOMAIN 93 173 PUA.
 DOMAIN 491 564 SUI1.
 MOD_RES 237 237 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Initiation factor; Phosphoprotein; Polymorphism; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 584 AA 
Protein Sequence
MFAKAFRVKS NTAIKGSDRR KLRADVTTAF PTLGTDQVSE LVPGKEELNI VKLYAHKGDA 60
VTVYVSGGNP ILFELEKNLY PTVYTLWSYP DLLPTFTTWP LVLEKLVGGA DLMLPGLVMP 120
PAGLPQVQKG DLCAISLVGN RAPVAIGVAA MSTAEMLTSG LKGRGFSVLH TYQDHLWRSG 180
NKSSPPSIAP LALDSADLSE EKGSVQMDST LQGDMRHMTL EGEEENGEVH QAREDKSLSE 240
APEDTSTRGL NQDSTDSKTL QEQMDELLQQ CFLHALKCRV KKADLPLLTS TFLGSHMFSC 300
CPEGRQLDIK KSSYKKLSKF LQQMQQEQII QVKELSKGVE SIVAVDWKHP RITSFVIPEP 360
SPTSQTIQEG SREQPYHPPD IKPLYCVPAS MTLLFQESGH KKGSFLEGSE VRTIVINYAK 420
KNDLVDADNK NLVRLDPILC DCILEKNEQH TVMKLPWDSL LTRCLEKLQP AYQVTLPGQE 480
PIVKKGRICP IDITLAQRAS NKKVTVVRNL EAYGLDPYSV AAILQQRCQA STTVNPAPGA 540
KDSLQVQIQG NQVHHLGWLL LEEYQLPRKH IQGLEKALKP GKKK 584 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0004872; F:receptor activity; TAS:ProtInc.
 GO:0003743; F:translation initiation factor activity; IDA:UniProtKB.
 GO:0006886; P:intracellular protein transport; TAS:ProtInc. 
Interpro
 IPR002478; PUA.
 IPR015947; PUA-like_domain.
 IPR003121; SWIB_MDM2_domain.
 IPR001950; TIF_SUI1. 
Pfam
 PF01253; SUI1 
SMART
 SM00359; PUA 
PROSITE
 PS50890; PUA
 PS50296; SUI1 
PRINTS