CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012623
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NGFI-A-binding protein 2 
Protein Synonyms/Alias
 EGR-1-binding protein 2; Melanoma-associated delayed early response protein; Protein MADER 
Gene Name
 NAB2 
Gene Synonyms/Alias
 MADER 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
169SPLELGEKLSPLPGGubiquitination[1]
379GPPLKKLKQEVGEQSsumoylation[2]
517EGRRSSVKVEAEASRsumoylation[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] The transcription factor Krox20 is an E3 ligase that sumoylates its Nab coregulators.
 García-Gutiérrez P, Juárez-Vicente F, Gallardo-Chamizo F, Charnay P, García-Domínguez M.
 EMBO Rep. 2011 Sep 30;12(10):1018-23. [PMID: 21836637] 
Functional Description
 Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability (By similarity). 
Sequence Annotation
 REGION 35 113 NCD1.
 REGION 267 356 NCD2.
 REGION 353 384 Necessary for nuclear localization (By
 MOD_RES 6 6 Phosphoserine.
 MOD_RES 159 159 Phosphoserine.
 MOD_RES 162 162 Phosphoserine.
 MOD_RES 171 171 Phosphoserine (By similarity).
 CROSSLNK 379 379 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 517 517 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Alternative splicing; Complete proteome; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 525 AA 
Protein Sequence
MHRAPSPTAE QPPGGGDSAR RTLQPRLKPS ARAMALPRTL GELQLYRVLQ RANLLSYYET 60
FIQQGGDDVQ QLCEAGEEEF LEIMALVGMA TKPLHVRRLQ KALREWATNP GLFSQPVPAV 120
PVSSIPLFKI SETAGTRKGS MSNGHGSPGE KAGSARSFSP KSPLELGEKL SPLPGGPGAG 180
DPRIWPGRST PESDVGAGGE EEAGSPPFSP PAGGGVPEGT GAGGLAAGGT GGGPDRLEPE 240
MVRMVVESVE RIFRSFPRGD AGEVTSLLKL NKKLARSVGH IFEMDDNDSQ KEEEIRKYSI 300
IYGRFDSKRR EGKQLSLHEL TINEAAAQFC MRDNTLLLRR VELFSLSRQV ARESTYLSSL 360
KGSRLHPEEL GGPPLKKLKQ EVGEQSHPEI QQPPPGPESY VPPYRPSLEE DSASLSGESL 420
DGHLQAVGSC PRLTPPPADL PLALPAHGLW SRHILQQTLM DEGLRLARLV SHDRVGRLSP 480
CVPAKPPLAE FEEGLLDRCP APGPHPALVE GRRSSVKVEA EASRQ 525 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003714; F:transcription corepressor activity; TAS:ProtInc.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0001958; P:endochondral ossification; IEA:Compara.
 GO:0042552; P:myelination; IEA:Compara.
 GO:0016480; P:negative regulation of transcription from RNA polymerase III promoter; IDA:MGI.
 GO:0007399; P:nervous system development; TAS:ProtInc.
 GO:0045682; P:regulation of epidermis development; IEA:Compara.
 GO:0014037; P:Schwann cell differentiation; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR006989; NAB_co-repressor_dom.
 IPR006988; Nab_N. 
Pfam
 PF04904; NCD1
 PF04905; NCD2 
SMART
  
PROSITE
  
PRINTS