CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010854
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin carboxyl-terminal hydrolase 2 
Protein Synonyms/Alias
 Deubiquitinating enzyme 2; Ubiquitin thioesterase 2; Ubiquitin-specific-processing protease 2 
Gene Name
 UBP2 
Gene Synonyms/Alias
 YOR124C; O3281; YOR3281C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
980QLFYGTTKQSIVPLSubiquitination[1]
991VPLSATNKVRTKVERubiquitination[1]
1065RERLMPFKSIEPLPFubiquitination[1]
1145TIKKTPLKIEAANDVacetylation[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Has an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety in natural or engineered linear fusion proteins, irrespective of their size or the presence of an N-terminal extension to ubiquitin. Removes ubiquitin chains that initiate proteolysis of FZO1 and inhibit mitochondrial fusion. 
Sequence Annotation
 ACT_SITE 745 745 Nucleophile (By similarity).
 ACT_SITE 1209 1209 Proton acceptor (By similarity).
 MOD_RES 907 907 Phosphoserine.  
Keyword
 Complete proteome; Hydrolase; Phosphoprotein; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1272 AA 
Protein Sequence
MPNEDNELQK AIENHHNQLL NQDKENADRN GSVIEDLPLY GTSINQQSTP GDVDDGKHLL 60
YPDIATNLPL KTSDRLLDDI LCDTIFLNST DPKVMQKGLQ SRGILKESML SYSTFRSSIR 120
PNCLGSLTDQ VVFQTKSEYD SISCPKYNKI HVFQAVIFNP SLAEQQISTF DDIVKIPIYH 180
LKVSVKVRQE LERLKKHVGV TQFHSLDHLH EYDRVDLSTF DSSDPNLLDY GIYVSDDTNK 240
LILIEIFKPE FNSPEEHESF TADAIKKRYN AMCVKNESLD KSETPSQVDC FYTLFKIFKG 300
PLTRKSKAEP TKTIDSGNLA LNTHLNPEWL TSKYGFQASS EIDEETNEIF TEYVPPDMVD 360
YVNDLETRKI RESFVRKCLQ LIFWGQLSTS LLAPNSPLKN TKSVKGMSSL QTSFSTLPWF 420
HLLGESRARI LLNSNEQTHS PLDAEPHFIN LSVSHYYTDR DIIRNYESLS SLDPENIGLY 480
FDALTYIANR KGAYQLIAYC GKQDIIGQEA LENALLMFKI NPKECNISEL NEATLLSIYK 540
YETSNKSQVT SNHLTNLKNA LRLLAKYTKS DKLKFYVDHE PYRALSQAYD TLSIDESVDE 600
DIIKTAYSVK INDSPGLKLD CDRALYTIAI SKRSLDLFNF LTEECPQFSN YYGPEKLDYQ 660
EALKLLQVNE NASDETILKI FKQKWFDENV YEPDQFLILR AALTKISIER NSTLITNFLL 720
TGTIDPNSLP PENWPTGINN IGNTCYLNSL LQYYFSIAPL RRYVLEYQKT VENFNDHLSN 780
SGHIRRIGGR EISRGEVERS IQFIYQLRNL FYAMVHTRER CVTPSKELAY LAFAPSNVEV 840
EFEVEGNKVV DQTGVLSDSK KETTDDAFTT KIKDTSLIDL EMEDGLNGDV GTDANRKKNE 900
SNDAEVSENE DTTGLTSPTR VAKISSDQLE NALEMGRQQD VTECIGNVLF QIESGSEPIR 960
YDEDNEQYDL VKQLFYGTTK QSIVPLSATN KVRTKVERFL SLLINIGDHP KDIYDAFDSY 1020
FKDEYLTMEE YGDVIRTVAV TTFPTILQVQ IQRVYYDRER LMPFKSIEPL PFKEVIYMDR 1080
YADTENPLLL AKKKETEEMK QKLKVMKNRQ RELLSRDDSG LTRKDAFLES IKLLESDTIK 1140
KTPLKIEAAN DVIKTLRNNV QNIDNELMKL YNDINSLEEK ISHQFDDFKE YGYSLFSVFI 1200
HRGEASYGHY WIYIKDRNRN GIWRKYNDET ISEVQEEEVF NFNEGNTATP YFLVYVKQGQ 1260
EGDIEPLKRI LK 1272 
Gene Ontology
 GO:0005737; C:cytoplasm; IC:SGD.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro.
 GO:0004843; F:ubiquitin-specific protease activity; IDA:SGD.
 GO:0016579; P:protein deubiquitination; IDA:SGD.
 GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IMP:SGD. 
Interpro
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19.
 IPR025305; UCH_repeat_domain. 
Pfam
 PF13446; RPT
 PF00443; UCH 
SMART
  
PROSITE
 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3 
PRINTS