UniProt Accession

 HAOX1_MOUSE; Q9WU19 

Genbank Protein ID

 AF104312 

Genbank Nucleotide ID

 AAD25332.1 

Protein Name

 Hydroxyacid oxidase 1 

Protein Synonyms/Alias

 HAOX1; Glycolate oxidase; GOX 

Gene Name

 Hao1 

Gene Synonyms/Alias

 Gox1; Hao-1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
21	HVRSVLQKSVYDYYR	ubiquitination	[1]
184	LPPQLRMKNFETNDL	acetylation	[2]
184	LPPQLRMKNFETNDL	succinylation	[2]
184	LPPQLRMKNFETNDL	ubiquitination	[1]
236	TSLPIVVKGILRGDD	ubiquitination	[1]
245	ILRGDDAKEAVKHGV	acetylation	[3, 4]
249	DDAKEAVKHGVDGIL	ubiquitination	[1]
302	RKGTDVLKALALGAK	ubiquitination	[1]
337	QDVLEILKEEFRLAM	ubiquitination	[1]
353	LSGCQNVKVIDKTLV	acetylation	[2]
353	LSGCQNVKVIDKTLV	succinylation	[2]
357	QNVKVIDKTLVRKNP	acetylation	[2]
357	QNVKVIDKTLVRKNP	succinylation	[2]
357	QNVKVIDKTLVRKNP	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2- hydroxy octanoate (By similarity). 

Sequence Annotation

 DOMAIN 1 365 FMN hydroxy acid dehydrogenase.
 NP_BIND 291 315 FMN (By similarity).
 MOTIF 368 370 Microbody targeting signal (Potential).
 ACT_SITE 260 260 Proton acceptor (By similarity).
 BINDING 26 26 Substrate (Potential).
 BINDING 108 108 FMN (By similarity).
 BINDING 130 130 FMN (By similarity).
 BINDING 132 132 Substrate (By similarity).
 BINDING 158 158 FMN (By similarity).
 BINDING 167 167 Substrate (By similarity).
 BINDING 236 236 FMN (By similarity).
 BINDING 263 263 Substrate (Potential).
 BINDING 315 315 FMN (By similarity).  

Keyword

 Complete proteome; Flavoprotein; FMN; Oxidoreductase; Peroxisome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 370 AA 

Protein Sequence

Gene Ontology

 GO:0005777; C:peroxisome; IDA:HGNC.
 GO:0010181; F:FMN binding; ISS:UniProtKB.
 GO:0008891; F:glycolate oxidase activity; ISS:UniProtKB.
 GO:0052853; F:long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity; IEA:EC.
 GO:0052854; F:medium-chain-(S)-2-hydroxy-acid oxidase activity; IEA:EC.
 GO:0052852; F:very-long-chain-(S)-2-hydroxy-acid oxidase activity; IEA:EC.
 GO:0001561; P:fatty acid alpha-oxidation; ISS:UniProtKB.
 GO:0046296; P:glycolate catabolic process; ISS:UniProtKB.
 GO:0006979; P:response to oxidative stress; IEA:Compara. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR012133; Alpha-hydoxy_acid_DH_FMN.
 IPR000262; FMN-dep_DH.
 IPR008259; FMN_hydac_DH_AS. 

Pfam

 PF01070; FMN_dh 

SMART

  

PROSITE

 PS00557; FMN_HYDROXY_ACID_DH_1
 PS51349; FMN_HYDROXY_ACID_DH_2 

PRINTS