CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003900
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Breakpoint cluster region protein 
Protein Synonyms/Alias
 Renal carcinoma antigen NY-REN-26 
Gene Name
 BCR 
Gene Synonyms/Alias
 BCR1; D22S11 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
583RVGDLFQKLASQLGVubiquitination[1, 2]
645SLETLLYKPVDRVTRubiquitination[2]
662LVLHDLLKHTPASHPubiquitination[2]
1066VTKRERSKVPYIVRQubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity. 
Sequence Annotation
 DOMAIN 498 691 DH.
 DOMAIN 708 866 PH.
 DOMAIN 870 1002 C2.
 DOMAIN 1054 1248 Rho-GAP.
 REGION 1 426 Kinase.
 REGION 197 385 Binding to ABL SH2-domain.
 MOD_RES 122 122 Phosphoserine.
 MOD_RES 177 177 Phosphotyrosine; by HCK.
 MOD_RES 215 215 Phosphoserine.
 MOD_RES 236 236 Phosphoserine (By similarity).
 MOD_RES 246 246 Phosphotyrosine; by FES.
 MOD_RES 459 459 Phosphoserine.
 MOD_RES 1264 1264 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Chromosomal rearrangement; Complete proteome; GTPase activation; Guanine-nucleotide releasing factor; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1271 AA 
Protein Sequence
MVDPVGFAEA WKAQFPDSEP PRMELRSVGD IEQELERCKA SIRRLEQEVN QERFRMIYLQ 60
TLLAKEKKSY DRQRWGFRRA AQAPDGASEP RASASRPQPA PADGADPPPA EEPEARPDGE 120
GSPGKARPGT ARRPGAAASG ERDDRGPPAS VAALRSNFER IRKGHGQPGA DAEKPFYVNV 180
EFHHERGLVK VNDKEVSDRI SSLGSQAMQM ERKKSQHGAG SSVGDASRPP YRGRSSESSC 240
GVDGDYEDAE LNPRFLKDNL IDANGGSRPP WPPLEYQPYQ SIYVGGMMEG EGKGPLLRSQ 300
STSEQEKRLT WPRRSYSPRS FEDCGGGYTP DCSSNENLTS SEEDFSSGQS SRVSPSPTTY 360
RMFRDKSRSP SQNSQQSFDS SSPPTPQCHK RHRHCPVVVS EATIVGVRKT GQIWPNDGEG 420
AFHGDADGSF GTPPGYGCAA DRAEEQRRHQ DGLPYIDDSP SSSPHLSSKG RGSRDALVSG 480
ALESTKASEL DLEKGLEMRK WVLSGILASE ETYLSHLEAL LLPMKPLKAA ATTSQPVLTS 540
QQIETIFFKV PELYEIHKEF YDGLFPRVQQ WSHQQRVGDL FQKLASQLGV YRAFVDNYGV 600
AMEMAEKCCQ ANAQFAEISE NLRARSNKDA KDPTTKNSLE TLLYKPVDRV TRSTLVLHDL 660
LKHTPASHPD HPLLQDALRI SQNFLSSINE EITPRRQSMT VKKGEHRQLL KDSFMVELVE 720
GARKLRHVFL FTDLLLCTKL KKQSGGKTQQ YDCKWYIPLT DLSFQMVDEL EAVPNIPLVP 780
DEELDALKIK ISQIKNDIQR EKRANKGSKA TERLKKKLSE QESLLLLMSP SMAFRVHSRN 840
GKSYTFLISS DYERAEWREN IREQQKKCFR SFSLTSVELQ MLTNSCVKLQ TVHSIPLTIN 900
KEDDESPGLY GFLNVIVHSA TGFKQSSNLY CTLEVDSFGY FVNKAKTRVY RDTAEPNWNE 960
EFEIELEGSQ TLRILCYEKC YNKTKIPKED GESTDRLMGK GQVQLDPQAL QDRDWQRTVI 1020
AMNGIEVKLS VKFNSREFSL KRMPSRKQTG VFGVKIAVVT KRERSKVPYI VRQCVEEIER 1080
RGMEEVGIYR VSGVATDIQA LKAAFDVNNK DVSVMMSEMD VNAIAGTLKL YFRELPEPLF 1140
TDEFYPNFAE GIALSDPVAK ESCMLNLLLS LPEANLLTFL FLLDHLKRVA EKEAVNKMSL 1200
HNLATVFGPT LLRPSEKESK LPANPSQPIT MTDSWSLEVM SQVQVLLYFL QLEAIPAPDS 1260
KRQSILFSTE V 1271 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005096; F:GTPase activator activity; TAS:ProtInc.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0004674; F:protein serine/threonine kinase activity; TAS:ProtInc.
 GO:0004713; F:protein tyrosine kinase activity; TAS:Reactome.
 GO:0030675; F:Rac GTPase activator activity; IEA:Compara.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0030036; P:actin cytoskeleton organization; IEA:Compara.
 GO:0007420; P:brain development; IEA:Compara.
 GO:0042472; P:inner ear morphogenesis; IEA:Compara.
 GO:0030336; P:negative regulation of cell migration; IEA:Compara.
 GO:0050728; P:negative regulation of inflammatory response; IEA:Compara.
 GO:0043314; P:negative regulation of neutrophil degranulation; IEA:Compara.
 GO:0050885; P:neuromuscular process controlling balance; IEA:Compara.
 GO:0048008; P:platelet-derived growth factor receptor signaling pathway; IEA:Compara.
 GO:0050766; P:positive regulation of phagocytosis; IEA:Compara.
 GO:0046777; P:protein autophosphorylation; IEA:Compara.
 GO:0051726; P:regulation of cell cycle; IEA:Compara.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0032496; P:response to lipopolysaccharide; IEA:Compara.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome. 
Interpro
 IPR015123; Bcr-Abl_oncoprot_oligo.
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting.
 IPR000219; DH-domain.
 IPR001331; GDS_CDC24_CS.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom. 
Pfam
 PF09036; Bcr-Abl_Oligo
 PF00168; C2
 PF00169; PH
 PF00620; RhoGAP
 PF00621; RhoGEF 
SMART
 SM00239; C2
 SM00233; PH
 SM00324; RhoGAP
 SM00325; RhoGEF 
PROSITE
 PS50004; C2
 PS00741; DH_1
 PS50010; DH_2
 PS50003; PH_DOMAIN
 PS50238; RHOGAP 
PRINTS