UniProt Accession

 UB2E3_HUMAN; Q969T4; B2RAD6; D3DPG3; Q5U0R7; Q7Z4W4 

Genbank Protein ID

 AB017644; AF085362; AF136176; BT019345; AK314145; AC104076; CH471058; CH471058; BC003554; BC092407 

Genbank Nucleotide ID

 BAA76544.1; AAD40197.1; AAP97266.1; AAV38152.1; BAG36833.1; AAY14882.1; EAX10989.1; EAX10990.1; AAH03554.1; AAH92407.1 

Protein Name

 Ubiquitin-conjugating enzyme E2 E3 

Protein Synonyms/Alias

 UbcH9; Ubiquitin carrier protein E3; Ubiquitin-conjugating enzyme E2-23 kDa; Ubiquitin-protein ligase E3 

Gene Name

 UBE2E3 

Gene Synonyms/Alias

 UBCE4; UBCH9 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
68	TSAKRIQKELAEITL	ubiquitination	[1]
86	PNCSAGPKGDNIYEW	ubiquitination	[2]

Reference

 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest. 

Sequence Annotation

 ACT_SITE 145 145 Glycyl thioester intermediate.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 8 8 Phosphoserine.
 MOD_RES 91 91 Phosphotyrosine (By similarity).  

Keyword

 Acetylation; ATP-binding; Complete proteome; Cytoplasm; Growth regulation; Ligase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 207 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:UniProtKB.
 GO:0070979; P:protein K11-linked ubiquitination; IDA:UniProtKB.
 GO:0070936; P:protein K48-linked ubiquitination; IDA:UniProtKB.
 GO:0070534; P:protein K63-linked ubiquitination; IDA:UniProtKB.
 GO:0040008; P:regulation of growth; IEA:UniProtKB-KW. 

Interpro

 IPR000608; UBQ-conjugat_E2.
 IPR023313; UBQ-conjugating_AS.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF00179; UQ_con 

SMART

  

PROSITE

 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS