CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021871
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Misshapen-like kinase 1 
Protein Synonyms/Alias
 GCK family kinase MiNK; MAPK/ERK kinase kinase kinase 6; MEK kinase kinase 6; MEKKK 6; Misshapen/NIK-related kinase; Mitogen-activated protein kinase kinase kinase kinase 6 
Gene Name
 Mink1 
Gene Synonyms/Alias
 Map4k6; Mink 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
944KGQSPPTKDGGSDYQubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking. Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons. Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates: TANC1 upon stimulation by RAP2A, MBP and SMAD1. Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1. 
Sequence Annotation
 DOMAIN 25 289 Protein kinase.
 DOMAIN 995 1282 CNH.
 NP_BIND 31 39 ATP (By similarity).
 REGION 842 1308 Mediates interaction with RAP2A (By
 ACT_SITE 153 153 Proton acceptor (By similarity).
 BINDING 54 54 ATP (By similarity).
 MOD_RES 644 644 Phosphoserine (By similarity).
 MOD_RES 729 729 Phosphoserine.
 MOD_RES 745 745 Phosphoserine (By similarity).
 MOD_RES 746 746 Phosphoserine.
 MOD_RES 750 750 Phosphoserine.
 MOD_RES 892 892 Phosphoserine.
 MOD_RES 969 969 Phosphoserine.  
Keyword
 Alternative splicing; ATP-binding; Cell junction; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Postsynaptic cell membrane; Reference proteome; Serine/threonine-protein kinase; Synapse; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1308 AA 
Protein Sequence
MGDPAPARSL DDIDLSALRD PAGIFELVEV VGNGTYGQVY KGRHVKTGQL AAIKVMDVTE 60
DEEEEIKQEI NMLKKYSHHR NIATYYGAFI KKSPPGNDDQ LWLVMEFCGA GSVTDLVKNT 120
KGNALKEDCI AYICREILRG LAHLHAHKVI HRDIKGQNVL LTENAEVKLV DFGVSAQLDR 180
TVGRRNTFIG TPYWMAPEVI ACDENPDATY DYRSDIWSLG ITAIEMAEGA PPLCDMHPMR 240
ALFLIPRNPP PRLKSKKWSK KFTDFIDTCL IKTYLSRPPT EQLLKFPFIR DQPTERQVRI 300
QLKDHIDRSR KKRGEKEETE YEYSGSEEED DSHGEEGEPS SIMNVPGEST LRREFLRLQQ 360
ENKSNSEALK QQQQLQQQQQ RDPEAHIKHL LHQRQRRIEE QKEERRRVEE QQRREREQRK 420
LQEKEQQRRL EDMQALRREE ERRQAEREQE YKRKQLEEQR QSERLQRQLQ QEHAYLKSLQ 480
QQQQQQQLQK QQQQQQQILP GDRKPLYHYG RGINPADKPA WAREVEERAR MNKQQNSPLA 540
KAKPSSAGPE PPISQASPSP PGPLSQTPPM QRPVEPQEGP HKSLVAHRVP LKPYAAPVPR 600
SQSLQDQPTR NLAAFPASHD PDPAAVPTPT ATPSARGAVI RQNSDPTSEG PGPSPNPPSW 660
VRPDNEAPPK VPQRTSSIAT ALNTSGAGGS RPAQAVRARP RSNSAWQIYL QRRAERGTPK 720
PPGPPAQPPG PPNASSNPDL RRSDPGWERS DSVLPASHGH LPQAGSLERN RNRVGASTKL 780
DSSPVLSPGN KAKPEDHRSR PGRPASYKRA IGEDFVLLKE RTLDEAPKPP KKAMDYSSSS 840
EEVESSEEEE EEGDGEPSEG SRDTPGGRSD GDTDSVSTMV VHDVEEISGT QPSYGGGTMV 900
VQRTPEEERS LLLADSNGYT NLPDVVQPSH SPTENSKGQS PPTKDGGSDY QSRGLVKAPG 960
KSSFTMFVDL GIYQPGGSGD TIPITALVGG EGGRLDQLQF DVRKGSVVNV NPTNTRAHSE 1020
TPEIRKYKKR FNSEILCAAL WGVNLLVGTE NGLMLLDRSG QGKVYGLIGR RRFQQMDVLE 1080
GLNLLITISG KRNKLRVYYL SWLRNKILHN DPEVEKKQGW TTVGDMEGCG HYRVVKYERI 1140
KFLVIALKNS VEVYAWAPKP YHKFMAFKSF ADLPHRPLLV DLTVEEGQRL KVIYGSSAGF 1200
HAVDVDSGNS YDIYIPVHIQ SQITPHAIIF LPNTDGMEML LCYEDEGVYV NTYGRIIKDV 1260
VLQWGEMPTS VAYICSNQIM GWGEKAIEIR SVETGHLDGV FMHKRAQRLK FLCERNDKVF 1320
FASVRSGGSS QVYFMTLNRN CIMNW 1345 
Gene Ontology
 GO:0030424; C:axon; IEA:UniProtKB-SubCell.
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
 GO:0014069; C:postsynaptic density; IEA:UniProtKB-SubCell.
 GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-KW.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0005083; F:small GTPase regulator activity; IEA:InterPro.
 GO:0048813; P:dendrite morphogenesis; ISS:UniProtKB.
 GO:0007243; P:intracellular protein kinase cascade; IDA:UniProtKB.
 GO:0045060; P:negative thymic T cell selection; IMP:MGI.
 GO:0046777; P:protein autophosphorylation; ISS:UniProtKB.
 GO:2000311; P:regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity; ISS:UniProtKB.
 GO:0006950; P:response to stress; IDA:UniProtKB.
 GO:0007268; P:synaptic transmission; ISS:UniProtKB. 
Interpro
 IPR001180; Citron.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00780; CNH
 PF00069; Pkinase 
SMART
 SM00036; CNH
 SM00220; S_TKc 
PROSITE
 PS50219; CNH
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS