CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014648
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Plasma membrane calcium-transporting ATPase 4 
Protein Synonyms/Alias
 PMCA4; Plasma membrane calcium ATPase isoform 4; Plasma membrane calcium pump isoform 4 
Gene Name
 Atp2b4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
59QEICARLKTSPVEGLubiquitination[1]
75GNPADLEKRRLVFGKubiquitination[1]
252HVKKTLDKDPMLLSGubiquitination[1]
337EGLDSEEKEKKASKGubiquitination[1]
561RSEMPEEKLFKVYTFubiquitination[1]
607KCDRILNKEGGIVPFubiquitination[1]
735NRLIRNEKGEVEQEKubiquitination[1]
742KGEVEQEKLDKVWPRubiquitination[1]
745VEQEKLDKVWPRLRVubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. 
Sequence Annotation
 REGION 1086 1103 Calmodulin-binding subdomain A (By
 REGION 1104 1113 Calmodulin-binding subdomain B (By
 ACT_SITE 465 465 4-aspartylphosphate intermediate (By
 METAL 785 785 Magnesium (By similarity).
 METAL 789 789 Magnesium (By similarity).
 MOD_RES 1102 1102 Phosphothreonine; by PKC (By similarity).  
Keyword
 Alternative splicing; ATP-binding; Calcium; Calcium transport; Calmodulin-binding; Cell membrane; Complete proteome; Hydrolase; Ion transport; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1203 AA 
Protein Sequence
MTNPSGHNLP ANSVAESYEG EFGCTLMDLR KLMELRAADA VTQISAHYGS VQEICARLKT 60
SPVEGLSGNP ADLEKRRLVF GKNMIPPKKP KTFLELVWEA LQDVTLIILE IAAIISLVLS 120
FYRPPGGENE ICGHIVSNPE EDEEGETGWI EGAAILASVI IVVFVTAFND WSKEKQFRGL 180
QSRIELEQKF SIIRNGQLIQ LPVAEIVVGD IAQVKYGDLL PADGILIQGN DLKIDESSLT 240
GESDHVKKTL DKDPMLLSGT HVMEGSGRMV VTAVGINSQT GIIFTLLGAN EEEDDEKKKK 300
GKKQGVSENR NKAKTQDGVA LEIQPLNSQE GLDSEEKEKK ASKGPKKEKS VLQGKLTRLA 360
VQIGKAGLIM SILTVLILIL YFVVDNFVIQ RRAWLPECTP VYIQYFVKFF IIGVTVLVVA 420
VPEGLPLAVT ISLAYSVKKM MKDNNLVRHL DACETMGNAT AICSDKTGTL TMNRMTVVQA 480
YIGGTHYRQI PKPDDLPPNV LDLIVNSICI NSAYTSKILP PEKEGGLPRQ VGNKTECGLL 540
GFVTDLKQDY QAVRSEMPEE KLFKVYTFNS VRKSMSTVIR KPEGGFRVFS KGASEIMLRK 600
CDRILNKEGG IVPFKTKDRD NMVRNVIEPM ASEGLRTIGI AYRDFDGEEP SWENENEIFT 660
GLVCIAVVGI EDPVRPEVPD AINKCKRAGI TVRMVTGDNV NTARAIATKC GILTPGDDFL 720
CLEGKEFNRL IRNEKGEVEQ EKLDKVWPRL RVLARSSPTD KHTLVKGIID SNIGEQRQVV 780
AVTGDGTNDG PALKKADVGF AMGIAGTDVA KEASDIILTD DNFTSIVKAV MWGRNVYDSI 840
SKFLQFQLTV NVVAVIVAFS GACITQDSPL KAVQMLWVNL IMDTFASLAL ATEPPTDSLL 900
RRRPYGRNKP LISRTMMKNI LGHAVYQLGI VFLLVFAGDK LFDIDSGRKA PLNSPPSQHY 960
TIVFNTFVLM QLFNEINSRK IHGEKNVFAG VYRNIIFCSV VLGTFFCQIL IVEVGGKPFS 1020
CTNLTMEQWM WCLFIGIGEL LWGQVISAIP TKSLKFLKEA GHGSDKEEIS KDAEGLEEID 1080
HAEMELRRGQ ILWVRGLNRI QTQIEVINKF QTGASFKGVL RRQNLSQQLD VKLVPSSYSE 1140
AVASVRTSPS TSSAVTPPPV GNQSGQSIS 1169 
Gene Ontology
 GO:0016323; C:basolateral plasma membrane; IDA:RGD.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0043005; C:neuron projection; IDA:RGD.
 GO:0097228; C:sperm principal piece; IDA:RGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005388; F:calcium-transporting ATPase activity; IDA:RGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:1901660; P:calcium ion export; IMP:RGD.
 GO:0006874; P:cellular calcium ion homeostasis; TAS:RGD.
 GO:0021766; P:hippocampus development; IEP:RGD.
 GO:0003407; P:neural retina development; IEP:RGD.
 GO:0007283; P:spermatogenesis; IEP:RGD. 
Interpro
 IPR022141; ATP_Ca_trans_C.
 IPR006408; ATPase_P-typ_Ca-transp_plasma.
 IPR006068; ATPase_P-typ_cation-transptr_C.
 IPR004014; ATPase_P-typ_cation-transptr_N.
 IPR023299; ATPase_P-typ_cyto_domN.
 IPR018303; ATPase_P-typ_P_site.
 IPR023298; ATPase_P-typ_TM_dom.
 IPR008250; ATPase_P-typ_transduc_dom_A.
 IPR001757; Cation_transp_P_typ_ATPase.
 IPR023214; HAD-like_dom. 
Pfam
 PF12424; ATP_Ca_trans_C
 PF00689; Cation_ATPase_C
 PF00690; Cation_ATPase_N
 PF00122; E1-E2_ATPase
 PF00702; Hydrolase 
SMART
 SM00831; Cation_ATPase_N 
PROSITE
 PS00154; ATPASE_E1_E2 
PRINTS
 PR00119; CATATPASE.