| Tag | Content |
|---|
| CPLM ID | CPLM-013833 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | E3 ubiquitin-protein ligase NEDD4-like |
Protein Synonyms/Alias | |
Gene Name | NEDD4L |
Gene Synonyms/Alias | |
Created Date | July 27, 2013 |
Organism | Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) |
NCBI Taxa ID | 9601 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 446 | SAPLEGAKDSPVRRA | ubiquitination | [1] | | 623 | YRRIMSVKRPDVLKA | ubiquitination | [1] |
|
Reference | [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass. Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C. Nat Cell Biol. 2012 Oct;14(10):1089-98. [ PMID: 23000965] |
Functional Description | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2 (By similarity). |
Sequence Annotation | DOMAIN 11 113 C2.
DOMAIN 197 230 WW 1.
DOMAIN 369 402 WW 2.
DOMAIN 481 514 WW 3.
DOMAIN 532 565 WW 4.
DOMAIN 624 958 HECT.
ACT_SITE 926 926 Glycyl thioester intermediate (By
MOD_RES 306 306 Phosphothreonine (By similarity).
MOD_RES 322 322 Phosphothreonine (By similarity).
MOD_RES 346 346 Phosphoserine; by WNK1 and WNK4 (By
MOD_RES 430 430 Phosphoserine (By similarity).
MOD_RES 432 432 Phosphoserine; by SGK1 (By similarity).
MOD_RES 433 433 Phosphoserine; by WNK1 and WNK4 (By
MOD_RES 448 448 Phosphoserine; by SGK1 (By similarity).
MOD_RES 463 463 Phosphoserine (By similarity).
MOD_RES 467 467 Phosphoserine (By similarity).
MOD_RES 471 471 Phosphoserine (By similarity). |
Keyword | Complete proteome; Cytoplasm; Ligase; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation; Ubl conjugation pathway. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 959 AA |
Protein Sequence | MAPLSAALLP WHGVCVPVCY GESRILRVKV VSGIDLAKKD IFGASDPYVK LSLYVADENR 60
ELALVQTKTI KKTLNPKWNE EFYFRVNPSN HRLLFEVFDE NRLTRDDFLG QVDVPLSHLP 120
TEDPTMERPY TFKDFLLRPR SHKSRVKGFL RLKMAYMPKN GGQEEENSEQ RDDMEHGWEV 180
VDSNDSASQH QEELPPPPLP PGWEEKVDNL GRTYYVNHNN RTTQWHRPSL MDVSSESDNN 240
IRQINQEAAH RRFRSRRHIS EDLEPEPSEG GDVPEPWETI SEEVNIAGDS LGLALPPPPA 300
SPGSRTSPQE LSEELSRRLQ ITPDSNGEQF SSLIQREPSS RLRSCSVTDA VAEQGHLPPP 360
SVAYVHTTPG LPSGWEERKD AKGRTYYVNH NNRTTTWTRP IMQLAEDGAS GSATNSNNHL 420
IEPQIRRPRS LSSPTVTLSA PLEGAKDSPV RRAVKDTLSN PQSPQPSPYN SPKPQHKVTQ 480
SFLPPGWEMR IAPNGRPFFI DHNTKTTTWE DPRLKFPVHM RSKTSLNPND LGPLPPGWEE 540
RIHLDGRTFY IDHNSKITQW EDPRLQNPAI TGPAVPYSRE FKQKYDYFRK KLKKPADIPN 600
RFEMKLHRNN IFEESYRRIM SVKRPDVLKA RLWIEFESEK GLDYGGVARE WFFLLSKEMF 660
NPYYGLFEYS ATDNYTLQIN PNSGLCNEDH LSYFTFIGRV AGLAVFHGKL LDGFFIRPFY 720
KMMLGKQITL NDMESVDSEY YNSLKWILEN DPTELDLMFC IDEENFGQTY QVDLEPNGSE 780
IMVTNENKRE YIDLVIQWRF VNRVQKQMNA FLEGFTELLP IDLIKIFDEN ELELLMCGLG 840
DVDVNDWRQH SIYKNGYCPN HPVIQWFWKA VLLMDAEKRI RLLQFVTGTS RVPMNGFAEL 900
YGSNGPQLFT IEQWGSPEKL PRAHTCFNRL DLPPYETFED LREKLLMAVE NAQGFEGVD 959 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0004842; F:ubiquitin-protein ligase activity; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |