CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015855
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin thioesterase OTUB1 
Protein Synonyms/Alias
 Deubiquitinating enzyme OTUB1; OTU domain-containing ubiquitin aldehyde-binding protein 1; Otubain-1; Ubiquitin-specific-processing protease OTUB1 
Gene Name
 Otub1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
59LELSVLYKEYAEDDNubiquitination[1]
71DDNIYQQKIKDLHKKubiquitination[1]
84KKYSYIRKTRPDGNCubiquitination[1]
109EALLDDSKELQRFKAubiquitination[1]
188GYLQRESKFFEHFIEubiquitination[1]
201IEGGRTVKEFCQQEVubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Hydrolase that can specifically remove compared to 'Lys- 48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'- linked ubiquitin. 
Sequence Annotation
 DOMAIN 80 271 OTU.
 REGION 130 138 Ubiquitin-conjugating enzyme E2 binding
 REGION 169 177 Ubiquitin-conjugating enzyme E2 binding
 REGION 189 195 Free ubiquitin binding (By similarity).
 REGION 206 213 Ubiquitin-conjugating enzyme E2 binding
 REGION 214 221 Free ubiquitin binding (By similarity).
 REGION 245 251 Free ubiquitin binding (By similarity).
 ACT_SITE 88 88 By similarity.
 ACT_SITE 91 91 Nucleophile (By similarity).
 ACT_SITE 265 265 By similarity.
 BINDING 221 221 Free ubiquitin (By similarity).
 BINDING 235 235 Free ubiquitin (By similarity).
 BINDING 237 237 Free ubiquitin (By similarity).
 BINDING 261 261 Free ubiquitin (By similarity).
 BINDING 266 266 Free ubiquitin (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 16 16 Phosphoserine.  
Keyword
 Acetylation; Adaptive immunity; Complete proteome; Cytoplasm; Direct protein sequencing; DNA damage; DNA repair; Hydrolase; Immunity; Phosphoprotein; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 271 AA 
Protein Sequence
MAAEEPQQQK QEPLGSDSEG VNCLAYDEAI MAQQDRIQQE IAVQNPLVSE RLELSVLYKE 60
YAEDDNIYQQ KIKDLHKKYS YIRKTRPDGN CFYRAFGFSH LEALLDDSKE LQRFKAVSAK 120
SKEDLVSQGF TEFTIEDFHN TFMDLIEQVE KQTSVADLLA SFNDQSTSDY LVVYLRLLTS 180
GYLQRESKFF EHFIEGGRTV KEFCQQEVEP MCKESDHIHI IALAQALSVS IQVEYMDRGE 240
GGTTNPHVFP EGSEPKVYLL YRPGHYDILY K 271 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0019784; F:NEDD8-specific protease activity; ISS:UniProtKB.
 GO:0008242; F:omega peptidase activity; IEA:InterPro.
 GO:0043130; F:ubiquitin binding; ISS:UniProtKB.
 GO:0004843; F:ubiquitin-specific protease activity; ISS:UniProtKB.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:2000780; P:negative regulation of double-strand break repair; ISS:UniProtKB.
 GO:1901315; P:negative regulation of histone H2A K63-linked ubiquitination; ISS:UniProtKB.
 GO:0071108; P:protein K48-linked deubiquitination; ISS:UniProtKB.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0006974; P:response to DNA damage stimulus; ISS:UniProtKB. 
Interpro
 IPR003323; OTU.
 IPR019400; Peptidase_C65_otubain.
 IPR016615; Ubiquitin_thioesterase_Otubain. 
Pfam
 PF10275; Peptidase_C65 
SMART
  
PROSITE
 PS50802; OTU 
PRINTS