UniProt Accession

 IDHP_YEAST; P21954; D6VRT1 

Genbank Protein ID

 M57229; Z74114; BK006938 

Genbank Nucleotide ID

 AAA34703.1; CAA98631.1; DAA11791.1 

Protein Name

 Isocitrate dehydrogenase [NADP], mitochondrial 

Protein Synonyms/Alias

 IDH; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase 

Gene Name

 IDP1 

Gene Synonyms/Alias

 YDL066W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
41	MTRIIWDKIKKKLIL	acetylation	[1]
71	SRDATSDKITQDAAE	acetylation	[1]
100	TPDEARVKEFNLHKM	acetylation	[1]
106	VKEFNLHKMWKSPNG	acetylation	[1]
109	FNLHKMWKSPNGTIR	acetylation	[1]
142	RLVPRWEKPIIIGRH	acetylation	[1]
156	HAHGDQYKATDTLIP	acetylation	[1]
156	HAHGDQYKATDTLIP	ubiquitination	[2]
173	GSLELVYKPSDPTTA	acetylation	[1]
173	GSLELVYKPSDPTTA	ubiquitination	[2]
241	KKYDGRFKDIFQEVY	acetylation	[1]
253	EVYEAQYKSKFEQLG	acetylation	[1]
255	YEAQYKSKFEQLGIH	acetylation	[1]
279	VAQMIKSKGGFIMAL	acetylation	[1]
418	EFLDAVEKRLQKEIK	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 

Functional Description

 Mitochondrial IDP1 may regulate flux through the tricarboxylic acid cycle and respiration. Its probably critical function is the production of NADPH. 

Sequence Annotation

 NP_BIND 91 93 NADP (By similarity).
 NP_BIND 327 332 NADP (By similarity).
 REGION 110 116 Substrate binding (By similarity).
 METAL 269 269 Magnesium or manganese (By similarity).
 METAL 292 292 Magnesium or manganese (By similarity).
 BINDING 93 93 Substrate (By similarity).
 BINDING 98 98 NADP (By similarity).
 BINDING 125 125 Substrate (By similarity).
 BINDING 148 148 Substrate (By similarity).
 BINDING 277 277 NADP (By similarity).
 BINDING 345 345 NADP; via amide nitrogen and carbonyl  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; Glyoxylate bypass; Magnesium; Manganese; Metal-binding; Mitochondrion; NADP; Oxidoreductase; Reference proteome; Transit peptide; Tricarboxylic acid cycle. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 428 AA 

Protein Sequence

Gene Ontology

 GO:0042645; C:mitochondrial nucleoid; IDA:SGD.
 GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; TAS:SGD.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0006537; P:glutamate biosynthetic process; IMP:SGD.
 GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
 GO:0006102; P:isocitrate metabolic process; TAS:SGD.
 GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW. 

Interpro

 IPR019818; IsoCit/isopropylmalate_DH_CS.
 IPR004790; Isocitrate_DH_NADP.
 IPR024084; IsoPropMal-DH-like_dom. 

Pfam

 PF00180; Iso_dh 

SMART

  

PROSITE

 PS00470; IDH_IMDH 

PRINTS