CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009102
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Coronin-7 
Protein Synonyms/Alias
 Crn7; 70 kDa WD repeat tumor rejection antigen homolog 
Gene Name
 CORO7 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
103GSADRTVKLWRLPGPubiquitination[1]
157VKVWDAAKQQPLTELubiquitination[1]
188ALVGTACKDKQLRIFubiquitination[1, 2, 3]
190VGTACKDKQLRIFDPubiquitination[1]
241QMREREVKLWDTRFFubiquitination[1]
348IGYHVPRKAVEFHEDubiquitination[1]
472SLLGPSSKFRHAQGTacetylation[4]
472SLLGPSSKFRHAQGTubiquitination[1, 3, 5, 6, 7]
491DSHITNLKGLNLTTPubiquitination[1, 2, 3]
508SDGFCANKLRVAVPLubiquitination[1, 2, 3]
529VAVLELRKPGRLPDTubiquitination[1]
596VLTGHTEKICSLRFHubiquitination[1, 2, 3]
657QQLATVCKDGRVRVYubiquitination[1]
680LQEGPGPKGGRGARIubiquitination[1, 2, 3]
787KGLVLLPKTECDVREubiquitination[1]
892EEKSDQQKKEELLNAubiquitination[1]
893EKSDQQKKEELLNAMubiquitination[1]
903LLNAMVAKLGNREDPubiquitination[1, 5, 7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 May play a role in the maintenance of the Golgi apparatus morphology and in the protein export from the Golgi. 
Sequence Annotation
 REPEAT 75 115 WD 1.
 REPEAT 124 163 WD 2.
 REPEAT 166 205 WD 3.
 REPEAT 209 253 WD 4.
 REPEAT 542 582 WD 5.
 REPEAT 592 632 WD 6.
 REPEAT 635 674 WD 7.
 REPEAT 728 768 WD 8.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Golgi apparatus; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 925 AA 
Protein Sequence
MNRFRVSKFR HTEARPPRRE SWISDIRAGT APSCRNHIKS SCSLIAFNSD RPGVLGIVPL 60
QGQGEDKRRV AHLGCHSDLV TDLDFSPFDD FLLATGSADR TVKLWRLPGP GQALPSAPGV 120
VLGPEDLPVE VLQFHPTSDG ILVSAAGTTV KVWDAAKQQP LTELAAHGDL VQSAVWSRDG 180
ALVGTACKDK QLRIFDPRTK PRASQSTQAH ENSRDSRLAW MGTWEHLVST GFNQMREREV 240
KLWDTRFFSS ALASLTLDTS LGCLVPLLDP DSGLLVLAGK GERQLYCYEV VPQQPALSPV 300
TQCVLESVLR GAALVPRQAL AVMSCEVLRV LQLSDTAIVP IGYHVPRKAV EFHEDLFPDT 360
AGCVPATDPH SWWAGDNQQV QKVSLNPACR PHPSFTSCLV PPAEPLPDTA QPAVMETPVG 420
DADASEGFSS PPSSLTSPST PSSLGPSLSS TSGIGTSPSL RSLQSLLGPS SKFRHAQGTV 480
LHRDSHITNL KGLNLTTPGE SDGFCANKLR VAVPLLSSGG QVAVLELRKP GRLPDTALPT 540
LQNGAAVTDL AWDPFDPHRL AVAGEDARIR LWRVPAEGLE EVLTTPETVL TGHTEKICSL 600
RFHPLAANVL ASSSYDLTVR IWDLQAGADR LKLQGHQDQI FSLAWSPDGQ QLATVCKDGR 660
VRVYRPRSGP EPLQEGPGPK GGRGARIVWV CDGRCLLVSG FDSQSERQLL LYEAEALAGG 720
PLAVLGLDVA PSTLLPSYDP DTGLVLLTGK GDTRVFLYEL LPESPFFLEC NSFTSPDPHK 780
GLVLLPKTEC DVREVELMRC LRLRQSSLEP VAFRLPRVRK EFFQDDVFPD TAVIWEPVLS 840
AEAWLQGANG QPWLLSLQPP DMSPVSQAPR EAPARRAPSS AQYLEEKSDQ QKKEELLNAM 900
VAKLGNREDP LPQDSFEGVD EDEWD 925 
Gene Ontology
 GO:0016023; C:cytoplasmic membrane-bounded vesicle; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; ISS:UniProtKB. 
Interpro
 IPR027331; CORO7.
 IPR015505; Coronin.
 IPR015049; DUF1900.
 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF08954; DUF1900
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.