CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010588
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Vacuolar fusion protein CCZ1 homolog B 
Protein Synonyms/Alias
 Vacuolar fusion protein CCZ1 homolog-like 
Gene Name
 CCZ1B 
Gene Synonyms/Alias
 C7orf28B 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
44REGQEENKILFYHPNubiquitination[1]
55YHPNEVEKNEKIRNVubiquitination[1]
79TRTFSPSKPAKSLHTubiquitination[1]
88AKSLHTQKNRQFFNEubiquitination[2]
113VRNPIIEKQSKDGKPubiquitination[1, 2, 3, 4, 5]
116PIIEKQSKDGKPVIEubiquitination[1, 2]
119EKQSKDGKPVIEYQEubiquitination[1, 2, 3, 4]
132QEEELLDKVYSSVLRubiquitination[1]
162AMEDGGVKLLKERLEubiquitination[1]
170LLKERLEKFFHRYLQacetylation[6]
246DDMRILYKYLTTSLFubiquitination[1, 7]
295NLNDPDAKCRFPKIFubiquitination[1, 7, 8, 9]
372KRMSGSEKEPQFKFIubiquitination[1, 9]
389NHMNLAEKSTVHMRKubiquitination[1]
396KSTVHMRKTPSVSLTubiquitination[1, 7, 9]
443DYWVVGKKSDRRELYubiquitination[1]
456LYVILNQKNANLIEVubiquitination[1]
468IEVNEEVKKLCATQFubiquitination[1, 4]
469EVNEEVKKLCATQFNubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [8] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 266 266 Phosphoserine.  
Keyword
 Acetylation; Complete proteome; Lysosome; Membrane; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 482 AA 
Protein Sequence
MAAAAAGAGS GPWAAQEKQF PPALLSFFIY NPRFGPREGQ EENKILFYHP NEVEKNEKIR 60
NVGLCEAIVQ FTRTFSPSKP AKSLHTQKNR QFFNEPEENF WMVMVVRNPI IEKQSKDGKP 120
VIEYQEEELL DKVYSSVLRQ CYSMYKLFNG TFLKAMEDGG VKLLKERLEK FFHRYLQTLH 180
LQSCDLLDIF GGISFFPLDK MTYLKIQSFI NRMEESLNIV KYTAFLYNDQ LIWSGLEQDD 240
MRILYKYLTT SLFPRHIEPE LAGRDSPIRA EMPGNLQHYG RFLTGPLNLN DPDAKCRFPK 300
IFVNTDDTYE ELHLIVYKAM SAAVCFMIDA SVHPTLDFCR RLDSIVGPQL TVLASDICEQ 360
FNINKRMSGS EKEPQFKFIY FNHMNLAEKS TVHMRKTPSV SLTSVHPDLM KILGDINSDF 420
TRVDEDEEII VKAMSDYWVV GKKSDRRELY VILNQKNANL IEVNEEVKKL CATQFNNIFF 480
LD 482 
Gene Ontology
 GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell. 
Interpro
 IPR013176; DUF1712_fun. 
Pfam
 PF08217; DUF1712 
SMART
  
PROSITE
  
PRINTS