CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030549
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Very-long-chain enoyl-CoA reductase 
Protein Synonyms/Alias
 cDNA FLJ36768 fis, clone ADIPS1000064, highly similar to Synaptic glycoprotein SC2 
Gene Name
 TECR 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
27RVEILDAKTREKLCFubiquitination[1]
31LDAKTREKLCFLDKVubiquitination[2]
37EKLCFLDKVEPHATIubiquitination[2, 3]
48HATIAEIKNLFTKTHubiquitination[3, 4]
53EIKNLFTKTHPQWYPubiquitination[2, 3, 4, 5]
72LRLDPKGKSLKDEDVubiquitination[2, 4]
75DPKGKSLKDEDVLQKacetylation[6, 7]
75DPKGKSLKDEDVLQKubiquitination[4]
82KDEDVLQKLPVGTTAubiquitination[3, 4]
131VPFIYGHKYDFTSSRubiquitination[3]
239DLRPAGSKTRKIPYPubiquitination[1, 4]
306GKHRSYLKEFRDYPPubiquitination[1, 2, 3, 4, 5, 7, 8]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Membrane; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 323 AA 
Protein Sequence
MILQETKAAC GIPAIPHCSR VEILDAKTRE KLCFLDKVEP HATIAEIKNL FTKTHPQWYP 60
ARQSLRLDPK GKSLKDEDVL QKLPVGTTAT LYFRDLGAQI SWVTVFLTEY AGPLFIYLLF 120
YFRVPFIYGH KYDFTSSRHT VVHLACICHS FHYIKRLLET LFVHRFSHGT MPLRNIFKNC 180
TYYWGFAAWM AYYINHPLYT PPTYGAQQVK LALAIFVICQ LGNFSIHMAL RDLRPAGSKT 240
RKIPYPTKNP FTWLFLLVSC PNYTYEVGSW IGFAIMTQCL PVALFSLVGF TQMTIWAKGK 300
HRSYLKEFRD YPPLRMPIIP FLL 323 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0016627; F:oxidoreductase activity, acting on the CH-CH group of donors; IEA:InterPro.
 GO:0006629; P:lipid metabolic process; IEA:InterPro. 
Interpro
 IPR001104; 3-oxo-5_a-steroid_4-DH_C. 
Pfam
 PF02544; Steroid_dh 
SMART
  
PROSITE
 PS50244; S5A_REDUCTASE 
PRINTS