UniProt Accession

 NGG1_YEAST; P32494; D6VSF8 

Genbank Protein ID

 L12137; L21189; Z46727; BK006938 

Genbank Nucleotide ID

 AAA21684.1; AAA03542.1; CAA86681.1; DAA12018.1 

Protein Name

 Chromatin-remodeling complexes subunit NGG1 

Protein Synonyms/Alias

 Transcriptional adapter 3 

Gene Name

 NGG1 

Gene Synonyms/Alias

 ADA3; SWI7; YDR176W; YD9395.09 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
15	GKLPKGEKLPKKEGG	acetylation	[1]
51	DIGMLNGKSVRSIPN	acetylation	[1]
183	HRDQPREKRPFDSET	acetylation	[1]
668	FDKPEIMKRIPNESV	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Transcription regulator. Could inhibit GAL4 DNA-binding or its ability to activate transcription. Functions as component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA, SLIK and ADA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus. ADA preferentially acetylates nucleosomal histones H3 (at 'Lys-14' and 'Lys-18') and H2B. 

Sequence Annotation

 MOTIF 606 618 Nuclear localization signal (Potential).
 MOD_RES 134 134 Phosphoserine.
 MOD_RES 407 407 Phosphoserine.
 MOD_RES 464 464 Phosphothreonine.  

Keyword

 Chromatin regulator; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 702 AA 

Protein Sequence

Gene Ontology

 GO:0005671; C:Ada2/Gcn5/Ada3 transcription activator complex; IDA:SGD.
 GO:0000124; C:SAGA complex; IDA:SGD.
 GO:0046695; C:SLIK (SAGA-like) complex; IDA:SGD.
 GO:0003712; F:transcription cofactor activity; TAS:SGD.
 GO:0016573; P:histone acetylation; IDA:SGD.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR019340; Histone_AcTrfase_su3. 

Pfam

 PF10198; Ada3 

SMART

  

PROSITE

  

PRINTS