CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013197
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 SUMO-conjugating enzyme SCE1 
Protein Synonyms/Alias
 Protein EMBRYO DEFECTIVE 1637; Protein hus5 homolog; SUMO-conjugating enzyme 1; AtSCE1 
Gene Name
 SCE1 
Gene Synonyms/Alias
 AHUS5; EMB1637; At3g57870; T10K17.80 
Created Date
 July 27, 2013 
Organism
 Arabidopsis thaliana (Mouse-ear cress) 
NCBI Taxa ID
 3702 
Lysine Modification
Position
Peptide
Type
References
154KRVKLQSKQYPALV*sumoylation[1]
Reference
 [1] Proteomic analyses identify a diverse array of nuclear processes affected by small ubiquitin-like modifier conjugation in Arabidopsis.
 Miller MJ, Barrett-Wilt GA, Hua Z, Vierstra RD.
 Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16512-7. [PMID: 20813957
Functional Description
 SUMO-conjugating enzyme that accepts the SUMO proteins from the E1 SUMO-activating heterodimer SAE1/SAE2 and catalyzes its covalent attachment to other proteins with the E3 SUMO ligases SIZ1 and MMS21. Associates with SIZ1 for sumoylation of the transcription factor GTE3. 
Sequence Annotation
 ACT_SITE 94 94 Glycyl thioester intermediate.  
Keyword
 ATP-binding; Complete proteome; Ligase; Nucleotide-binding; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 160 AA 
Protein Sequence
MASGIARGRL AEERKSWRKN HPHGFVAKPE TGQDGTVNLM VWHCTIPGKA GTDWEGGFFP 60
LTMHFSEDYP SKPPKCKFPQ GFFHPNVYPS GTVCLSILNE DYGWRPAITV KQILVGIQDL 120
LDTPNPADPA QTDGYHLFCQ DPVEYKKRVK LQSKQYPALV 160 
Gene Ontology
 GO:0005634; C:nucleus; IDA:TAIR.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0019789; F:SUMO ligase activity; IDA:TAIR.
 GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
 GO:0016925; P:protein sumoylation; IDA:TAIR.
 GO:0009737; P:response to abscisic acid stimulus; IMP:TAIR. 
Interpro
 IPR027230; Ubc9.
 IPR000608; UBQ-conjugat_E2.
 IPR023313; UBQ-conjugating_AS.
 IPR016135; UBQ-conjugating_enzyme/RWD. 
Pfam
 PF00179; UQ_con 
SMART
  
PROSITE
 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 
PRINTS