CPLM-015240

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-015240

UniProt Accession

GAPD1_MOUSE; Q6PAR5; A0PJI8; A2AR09; A2AR10; A2AR17; A2AR18; Q3TNS1; Q3UDL0; Q3UYD5; Q6ZPP0; Q80V37; Q80ZK4; Q8BTS5; Q9CRS2; Q9CTI1

Genbank Protein ID

EF155419; AK129381; AK014474; AK088851; AK003521; AK134776; AK150026; AK165047; AL845262; AL929106; AL845262; AL929106; AL845262; AL929106; AL845262; AL845262; AL929106; AL845262; AL929106; AL845262; AL929106; AL845262; BC031478; BC043715; BC048847; BC057164; BC060123

Genbank Nucleotide ID

ABM68541.1; BAC98191.1; BAB29377.2; BAC40613.1; BAB22834.1; BAE22277.1; BAE29251.1; BAE38017.1; CAM15445.1; CAM15445.1; CAM15446.1; CAM15446.1; CAM15447.1; CAM15447.1; CAM15455.1; CAM15456.1; CAM24604.1; CAM24604.1; CAM24605.1; CAM24605.1; CAM24606.1; CAM24606.1; AAH31478.1; AAH43715.1; AAH48847.1; AAH57164.1; AAH60123.1

Protein Name

GTPase-activating protein and VPS9 domain-containing protein 1

Protein Synonyms/Alias

GAPex-5; Rab5-activating protein 6

Gene Name

Gapvd1

Gene Synonyms/Alias

Gapex5; Kiaa1521

Created Date

July 27, 2013

Organism

Mus musculus (Mouse)

NCBI Taxa ID

10090

Lysine Modification

Position	Peptide	Type	References
24	RLYVSSEKQLIQRLN	ubiquitination	[1]
49	YRTAWIAKQQRINLD	ubiquitination	[1]
1313	SVMNRIFKLAFYPNQ	ubiquitination	[1]

Reference

[1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]

Functional Description

Acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), and participates in various processes such as endocytosis, insulin receptor internalization or LC2A4/GLUT4 trafficking. Acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, leading to regulate LC2A4/GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside the cells. Upon insulin stimulation, it is translocated to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles. Also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome. Has GEF activity for Rab5 and GAP activity for Ras.

Sequence Annotation

DOMAIN 131 353 Ras-GAP.
DOMAIN 1318 1458 VPS9.
MOD_RES 390 390 Phosphothreonine (By similarity).
MOD_RES 458 458 Phosphothreonine (By similarity).
MOD_RES 466 466 Phosphoserine (By similarity).
MOD_RES 470 470 Phosphothreonine (By similarity).
MOD_RES 566 566 Phosphoserine (By similarity).
MOD_RES 766 766 Phosphoserine (By similarity).
MOD_RES 902 902 Phosphoserine.
MOD_RES 903 903 Phosphoserine.
MOD_RES 964 964 Phosphoserine (By similarity).
MOD_RES 1017 1017 Phosphoserine (By similarity).
MOD_RES 1076 1076 Phosphoserine (By similarity).
MOD_RES 1085 1085 Phosphoserine.

Keyword

Alternative splicing; Complete proteome; Endocytosis; Endosome; GTPase activation; Guanine-nucleotide releasing factor; Membrane; Phosphoprotein; Reference proteome.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

1458 AA

Protein Sequence

MVKLDIHTLA HHLKQERLYV SSEKQLIQRL NADVLKTAEK LYRTAWIAKQ QRINLDRLII 60
TSAEASPAEC CQHAKILEDT QFVDGYKQLG FQETAYGEFL SRLRENPRLI ASSLVAGEKL 120
NQENTQSVIY TVFTSLYGNC IMQEDESYLL QVLRYLIEFE LKESDNPRRL LRRGTCAFSI 180
LFKLFSEGLF SAKLFLTATL HEPIMQLLVE DEDHLETDPN KLIERFSPAQ QEKLFGEKGS 240
DRFRQKVQEM VDSNEAKLVA LVNKFIGYLK QNTYCFPHSL RWIVSQMYKT LSCVDRLEVG 300
EVRAMCTDLL LACFICPAVV NPEQYGIISD APINEVARFN LMQVGRLLQQ LAMTGTEEGD 360
PRTKNSLGKF DKGCVAAFLD VVIGGRAVET PPMSSVNLLE GLSRTVVYIS YSQLITLVNF 420
MKSVMSGDQL KEDRMALDNL LANLPQAKPG KSSSLDMTPY STPQMSPATT PANKKNRLPI 480
GQQLAAITAW DSSATNLTAH IPLVTPFATR SRSRSNMLMD LHMDHEGSSQ ETIQEVQPEE 540
VLVISLGTGP QLTPGMMSEN EVLNMQLSDG GQGDVPVDEN KLHGPSNRSN SVSSLDLEGE 600
SVSELGAGPS GSNGVEALQL LEHEQATTQD NLDDKLRKFE IRDMMGLTDD RDISETVSET 660
WSTDVLGSDF DPNVDEDRLQ EIAGAAAENV LGSLLCLPGS GSVLLDPCTG STISETTSEA 720
WSVEVLPSDS EAPDLKQEER LQELESCSGL GSTSDDTDVR EVSSRPSTPG LSVVSGISAT 780
SEDIPNKIED LRSECSSDFG GKDSVTSPDM DDIAHGAHQL TSPPSQSESL LAMFDPLSSH 840
EGASAVVRPK VHYARPSHPP PDPPILEGAV GGNEARLPNF GSHVLTAAEM EAFKQRHSYP 900
ERLVRSRSSD IVSSVRRPMS DPSWNRRPGN EELPPAAATG ATSLVAAPHS SSSSPSKDSS 960
RGETEERKDS DDERSDRSRP WWRKRFVSAM PKAPIPFRKK EKQEKDKDDL GPDRFSTLTD 1020
EPSPRLSAQA QVAEDILDKY RNAIKRTSPS EGAMANDESA EVMGDGESAH DSPREEALQN 1080
ISADDLPDSA SQAAHPQDSA FSYRDVKKKL RLALCSADSV AFPVLTHSTR NGLPDHTDPE 1140
DNEIVCFLKV QIAEAINLQD KSLMAQLQET MRCVCRFDNR TCRKLLASIA EDYRKRAPYI 1200
AYLTRCRQGL QTTQAHLERL LQRVLRDKEV ANRYFTTVCV RLLLESKEKK IREFIQDFQK 1260
LTAADDKTAQ VEDFLQFLYG VMAQDVIWQN ASEEQLQDAQ LAIERSVMNR IFKLAFYPNQ 1320
DGDILRDQVL HEHIQRLSKV VTANHRALQI PEVYLREAPW PSAQSEIRTI SAYKTPRDKV 1380
QCILRMCSTI MNLLSLANED SVPGADDFVP VLVFVLIKAN PPCLLSTVQY ISSFYASCLS 1440
GEESYWWMQF TAAVEFIKTI DDRK 1464

Gene Ontology

GO:0005829; C:cytosol; IDA:UniProtKB.
GO:0005768; C:endosome; IEA:UniProtKB-SubCell.
GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
GO:0032794; F:GTPase activating protein binding; IMP:UniProtKB.
GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
GO:0005085; F:guanyl-nucleotide exchange factor activity; IMP:UniProtKB.
GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
GO:0043547; P:positive regulation of GTPase activity; IEA:GOC.
GO:0051223; P:regulation of protein transport; IMP:UniProtKB.
GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro.
GO:0007165; P:signal transduction; IEA:InterPro.

Interpro

IPR001936; RasGAP.
IPR008936; Rho_GTPase_activation_prot.
IPR003123; VPS9.
IPR013995; VPS9_subgr.

Pfam

PF00616; RasGAP
PF02204; VPS9

SMART

SM00167; VPS9

PROSITE

PS00509; RAS_GTPASE_ACTIV_1
PS50018; RAS_GTPASE_ACTIV_2
PS51205; VPS9

PRINTS