UniProt Accession

 NCS1_MOUSE; Q8BNY6; A2AJ84 

Genbank Protein ID

 AF020184; AL732572; BC059825; AK079136 

Genbank Nucleotide ID

 AAD01642.1; CAM21076.1; AAH59825.1; BAC37557.1 

Protein Name

 Neuronal calcium sensor 1 

Protein Synonyms/Alias

 NCS-1; Frequenin homolog 

Gene Name

 Ncs1 

Gene Synonyms/Alias

 Freq 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
50	LDAAGFQKIYKQFFP	ubiquitination	[1]
53	AGFQKIYKQFFPFGD	ubiquitination	[1]
106	EKLRWAFKLYDLDND	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N- type voltage-gated calcium channel (By similarity). 

Sequence Annotation

 DOMAIN 24 59 EF-hand 1.
 DOMAIN 60 95 EF-hand 2.
 DOMAIN 96 131 EF-hand 3.
 DOMAIN 144 179 EF-hand 4.
 REGION 174 190 Interaction with IL1RAPL1 (By
 LIPID 2 2 N-myristoyl glycine (By similarity).  

Keyword

 Calcium; Cell junction; Cell membrane; Complete proteome; Cytoplasm; Golgi apparatus; Lipoprotein; Membrane; Metal-binding; Myristate; Postsynaptic cell membrane; Reference proteome; Repeat; Synapse. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 190 AA 

Protein Sequence

Gene Ontology

 GO:0030424; C:axon; IDA:MGI.
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0031410; C:cytoplasmic vesicle; IEA:Compara.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0030425; C:dendrite; IDA:MGI.
 GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0014069; C:postsynaptic density; IEA:UniProtKB-SubCell.
 GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-KW.
 GO:0005509; F:calcium ion binding; TAS:MGI.
 GO:0005245; F:voltage-gated calcium channel activity; ISS:UniProtKB.
 GO:0048015; P:phosphatidylinositol-mediated signaling; IEA:Compara.
 GO:0045921; P:positive regulation of exocytosis; IEA:Compara.
 GO:0050806; P:positive regulation of synaptic transmission; TAS:MGI.
 GO:0010975; P:regulation of neuron projection development; ISS:UniProtKB. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR001125; Recoverin. 

Pfam

 PF00036; efhand 

SMART

 SM00054; EFh 

PROSITE

 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 

PRINTS

 PR00450; RECOVERIN.