UniProt Accession

 NB5R3_RAT; P20070; Q64569; Q64720 

Genbank Protein ID

 D00636; J03867; BC062066; X65191; X65191; X65190; X77117 

Genbank Nucleotide ID

 BAA00530.1; AAA41008.1; AAH62066.1; CAA46308.1; CAA46309.1; CAA46307.1 

Protein Name

 NADH-cytochrome b5 reductase 3 

Protein Synonyms/Alias

 B5R; Cytochrome b5 reductase; Diaphorase-1; NADH-cytochrome b5 reductase 3 membrane-bound form; NADH-cytochrome b5 reductase 3 soluble form 

Gene Name

 Cyb5r3 

Gene Synonyms/Alias

 Dia1 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
25	FVYSLFMKLFQRSSP	acetylation	[1]
42	TLENPDIKYPLRLID	acetylation	[1]
50	YPLRLIDKEIISHDT	acetylation	[1]
115	LVVKVYFKDTHPKFP	acetylation	[1]
120	YFKDTHPKFPAGGKM	acetylation	[1]
156	LVYQGKGKFAIRADK	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. 

Sequence Annotation

 DOMAIN 40 152 FAD-binding FR-type.
 NP_BIND 132 147 FAD.
 NP_BIND 171 206 FAD.
 MOD_RES 42 42 N6-acetyllysine (By similarity).
 MOD_RES 43 43 Phosphotyrosine (By similarity).
 MOD_RES 120 120 N6-acetyllysine (By similarity).
 MOD_RES 130 130 Phosphotyrosine (By similarity).
 LIPID 2 2 N-myristoyl glycine.  

Keyword

 3D-structure; Acetylation; Alternative initiation; Alternative promoter usage; Cholesterol biosynthesis; Cholesterol metabolism; Complete proteome; Cytoplasm; Direct protein sequencing; Endoplasmic reticulum; FAD; Flavoprotein; Lipid biosynthesis; Lipid metabolism; Lipoprotein; Membrane; Mitochondrion; Mitochondrion outer membrane; Myristate; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 301 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IDA:HGNC.
 GO:0005811; C:lipid particle; IEA:Compara.
 GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
 GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
 GO:0043531; F:ADP binding; IDA:RGD.
 GO:0016208; F:AMP binding; IDA:RGD.
 GO:0004128; F:cytochrome-b5 reductase activity, acting on NAD(P)H; TAS:RGD.
 GO:0071949; F:FAD binding; IEA:Compara.
 GO:0050660; F:flavin adenine dinucleotide binding; IDA:RGD.
 GO:0051287; F:NAD binding; IDA:RGD.
 GO:0006695; P:cholesterol biosynthetic process; IEA:UniProtKB-KW. 

Interpro

 IPR017927; Fd_Rdtase_FAD-bd.
 IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
 IPR001834; NADH-Cyt_B5_reductase.
 IPR008333; OxRdtase_FAD-bd_dom.
 IPR001433; OxRdtase_FAD/NAD-bd.
 IPR017938; Riboflavin_synthase-like_b-brl. 

Pfam

 PF00970; FAD_binding_6
 PF00175; NAD_binding_1 

SMART

  

PROSITE

 PS51384; FAD_FR 

PRINTS

 PR00406; CYTB5RDTASE.
 PR00371; FPNCR.