CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002426
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Peptide chain release factor 2 
Protein Synonyms/Alias
 RF-2 
Gene Name
 prfB 
Gene Synonyms/Alias
 supK; b2891; JW5847 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
30GYLDYDAKKERLEEVacetylation[1]
58ERAQALGKERSSLEAacetylation[1]
162WAESRGFKTEIIEESacetylation[1]
162WAESRGFKTEIIEESpupylation[2]
284DRSQHKNKDQAMKQMacetylation[1]
294AMKQMKAKLYELEMQacetylation[1]
302LYELEMQKKNAEKQAacetylation[1]
307MQKKNAEKQAMEDNKacetylation[1]
314KQAMEDNKSDIGWGSacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli.
 Cerda-Maira FA, McAllister F, Bode NJ, Burns KE, Gygi SP, Darwin KH.
 EMBO Rep. 2011 Jul 8;12(8):863-70. [PMID: 21738222] 
Functional Description
 Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA. 
Sequence Annotation
 MOD_RES 252 252 N5-methylglutamine.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Methylation; Protein biosynthesis; Reference proteome; Ribosomal frameshifting. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 365 AA 
Protein Sequence
MFEINPVNNR IQDLTERSDV LRGYLDYDAK KERLEEVNAE LEQPDVWNEP ERAQALGKER 60
SSLEAVVDTL DQMKQGLEDV SGLLELAVEA DDEETFNEAV AELDALEEKL AQLEFRRMFS 120
GEYDSADCYL DIQAGSGGTE AQDWASMLER MYLRWAESRG FKTEIIEESE GEVAGIKSVT 180
IKISGDYAYG WLRTETGVHR LVRKSPFDSG GRRHTSFSSA FVYPEVDDDI DIEINPADLR 240
IDVYRTSGAG GQHVNRTESA VRITHIPTGI VTQCQNDRSQ HKNKDQAMKQ MKAKLYELEM 300
QKKNAEKQAM EDNKSDIGWG SQIRSYVLDD SRIKDLRTGV ETRNTQAVLD GSLDQFIEAS 360
LKAGL 365 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016149; F:translation release factor activity, codon specific; IDA:EcoCyc.
 GO:0015862; P:uridine transport; IMP:EcoliWiki. 
Interpro
 IPR014720; dsRNA-bd-like_dom.
 IPR005139; PCRF.
 IPR000352; Pep_chain_release_fac_I_II.
 IPR020853; Peptide_chain_release_fac2_bac.
 IPR004374; PrfB. 
Pfam
 PF03462; PCRF
 PF00472; RF-1 
SMART
 SM00937; PCRF 
PROSITE
 PS00745; RF_PROK_I 
PRINTS