UniProt Accession

 SIT1_HUMAN; Q9Y3P8; B2RBP9 

Genbank Protein ID

 AJ010059; AJ271888; AK314758; AL357874; CH471071; BC102029; BC104491; BC107484 

Genbank Nucleotide ID

 CAB41504.1; CAC81313.1; BAG37296.1; CAI13446.1; EAW58370.1; AAI02030.1; AAI04492.1; AAI07485.1 

Protein Name

 Signaling threshold-regulating transmembrane adapter 1 

Protein Synonyms/Alias

 SHP2-interacting transmembrane adapter protein; Suppression-inducing transmembrane adapter 1; gp30/40 

Gene Name

 SIT1 

Gene Synonyms/Alias

 SIT 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
147	PGPGTPVKYSEVVLD	ubiquitination	[1, 2, 3]

Reference

 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Negatively regulates TCR (T-cell antigen receptor)- mediated signaling in T-cells. Involved in positive selection of T-cells. 

Sequence Annotation

 REGION 90 93 Interaction with GRB2.
 REGION 146 151 Interaction with PTPN11.
 REGION 169 172 Interaction with CSK.
 REGION 188 191 Interaction with GRB2.
 MOD_RES 80 80 Phosphoserine.
 MOD_RES 90 90 Phosphotyrosine.
 MOD_RES 102 102 Phosphoserine.
 MOD_RES 127 127 Phosphotyrosine (Probable).
 MOD_RES 144 144 Phosphothreonine.
 MOD_RES 148 148 Phosphotyrosine.
 MOD_RES 169 169 Phosphotyrosine (Probable).
 MOD_RES 182 182 Phosphoserine.
 MOD_RES 188 188 Phosphotyrosine.
 CARBOHYD 26 26 N-linked (GlcNAc...).
 DISULFID 27 27 Interchain (Probable).  

Keyword

 Adaptive immunity; Cell membrane; Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; Immunity; Membrane; Phosphoprotein; Reference proteome; Signal; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 196 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005887; C:integral to plasma membrane; TAS:HGNC.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0042169; F:SH2 domain binding; TAS:HGNC.
 GO:0050863; P:regulation of T cell activation; IDA:UniProtKB.
 GO:0007165; P:signal transduction; TAS:ProtInc. 

Interpro

  

Pfam

  

SMART

  

PROSITE

  

PRINTS