CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032064
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Leupaxin 
Protein Synonyms/Alias
 cDNA FLJ51550, highly similar to Leupaxin 
Gene Name
 LPXN 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
66KESPPPSKTSAAAQLubiquitination[1]
86HLTEMQAKVAVRADAubiquitination[1, 2]
137GHCASCQKPIAGKVIubiquitination[3]
240DKKPYCRKDFLAMFSubiquitination[1, 4]
344FCLTQLSKGIFREQNubiquitination[1]
353IFREQNDKTYCQPCFubiquitination[1, 2, 5]
362YCQPCFNKLFPL***ubiquitination[1, 2, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; LIM domain; Metal-binding; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 366 AA 
Protein Sequence
MPYWRNWNAP PFRTVMNIPT QLLFPWISIP ERRLTLMRLR RSFLFRITQV PCREAQEPKE 60
SPPPSKTSAA AQLDELMAHL TEMQAKVAVR ADAGKKHLPD KQDHKASLDS MLGGLEQELQ 120
DLGIATVPKG HCASCQKPIA GKVIHALGQS WHPEHFVCTH CKEEIGSSPF FERSGLAYCP 180
NDYHQLFSPR CAYCAAPILD KVLTAMNQTW HPEHFFCSHC GEVFGAEGFH EKDKKPYCRK 240
DFLAMFSPKC GGCNRPVLEN YLSAMDTVWH PECFVCGDCF TSFSTGSFFE LDGRPFCELH 300
YHHRRGTLCH GCGQPITGRC ISAMGYKFHP EHFVCAFCLT QLSKGIFREQ NDKTYCQPCF 360
NKLFPL 366 
Gene Ontology
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR001781; Znf_LIM. 
Pfam
 PF00412; LIM 
SMART
 SM00132; LIM 
PROSITE
 PS00478; LIM_DOMAIN_1
 PS50023; LIM_DOMAIN_2 
PRINTS